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货号 | bs-5193R-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Rabbit Anti-phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) antibody |
中文名称 | Rabbit Anti-phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) antibody |
产品标签 | 磷酸化抗体 |
研究领域 | 肿瘤,免疫学,神经生物学,信号转导,细胞凋亡,转录调节因子,激酶和磷酸酶 |
英文别名 | phospho-AKT1/AKT2/AKT3 (Tyr315+Tyr316+Tyr312); AKT1/AKT2/AKT3(phospho-Tyr315/316/312); AKT1/2/3 (phospho Y315 + Y316 + Y312); AKT 1; AKT; AKT1; AKT-1; AKT1_HUMAN; AKT 2; AKT2; AKT-2; AKT2_HUMAN; AKT 3; AKT3; AKT-3; AKT3_HUMAN; C AKT; cAKT; MGC9965; MGC996 |
反应物种(已验证) | Human,Mouse,Rat |
反应物种(预测) | Chicken,Dog,Pig,Cow,Rabbit,Sheep |
产品应用(已验证) | WB,IHC,FCM |
产品应用(可推荐) | IF,ELISA |
推荐稀释比例 | WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500, |
克隆类型 | 多克隆 |
抗体来源 | Rabbit |
理论分子量 | 56 |
细胞定位 | 细胞核,细胞浆,细胞膜 |
性状 | Liquid |
免疫原 | KLH conjugated Synthesised phosphopeptide derived from human AKT1 around the phosphorylation site of Tyr315 |
抗原表位 | PE(p-Y)LA |
亚型 | IgG |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Cytoplasm. Nucleus. Cell membrane. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane w |
Tissue | Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers dur |
SIMILARITY | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. |
SUBUNIT | Interacts (via the C-terminus) with CCDC88A (via its C-terminus). Interacts with GRB10; the interaction leads to GRB10 phosphorylation thus promoting YWHAE-binding. Interacts with AGAP2 (isoform 2/PIKE-A); the interaction occurs in the presence of guanine |
Function | AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine a |
Post-translational | O-GlcNAcylation at Thr-305 and Thr-312 inhibits activating phosphorylation at Thr-308 via disrupting the interaction between AKT1 and PDPK1. O-GlcNAcylation at Ser-473 also probably interferes with phosphorylation at this site. Phosphorylation on Thr-3 |
DISEASE | Defects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the mos |
SWISS | P31749 |
Gene ID | 207 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. |