货号 |
bs-2446R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-phospho-eIF4E (Ser209) antibody |
中文名称 |
Rabbit Anti-phospho-eIF4E (Ser209) antibody |
产品标签 |
磷酸化抗体 |
研究领域 |
免疫学,信号转导,细胞凋亡,转录调节因子 |
英文别名 |
eIF4E(phospho S209); eIF4E(phospho Ser209); p-eIF4E(Ser209); EIF4F; eIF-4E; eIF 4E; CBP; eIF 4F 25 kDa subunit; CBP; eIF 4E; eIF 4F 25 kDa subunit; EIF 4F; EIF4E1; EIF4EL1; EIF4F; Eukaryotic Translation Initiation Factor 4 E; Eukaryotic translation initia |
反应物种(已验证) |
Human,Mouse,Rat,Zebrafish |
反应物种(预测) |
Chicken,Dog,Pig,Cow,Horse,Rabbit |
产品应用(已验证) |
WB,IHC,FCM |
产品应用(可推荐) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=0.2ug/test,IF=1:100-500, |
克隆类型 |
多克隆 |
抗体来源 |
Rabbit |
理论分子量 |
24 |
细胞定位 |
细胞核,细胞浆 |
性状 |
Liquid |
免疫原 |
KLH conjugated Synthesised phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209 |
抗原表位 |
SG(p-S)TT |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
Cytoplasm, P-body. |
SIMILARITY |
Belongs to the eukaryotic initiation factor 4E family. |
SUBUNIT |
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF |
Function |
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome bi |
Post-translational |
Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. |
SWISS |
P06730 |
Gene ID |
1977 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk. |