货号 |
bs-3031R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-phospho-ASK1 (Thr845) antibody |
中文名称 |
Rabbit Anti-phospho-ASK1 (Thr845) antibody |
产品标签 |
磷酸化抗体 |
研究领域 |
肿瘤,细胞生物,免疫学,神经生物学,信号转导,细胞凋亡,激酶和磷酸酶 |
英文别名 |
ASK1 (phospho Thr845); Mitogen-activated protein kinase kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; Apoptosis signal-regulating kinase 1; ASK-1; MAP3K5; ASK1; MAPKKK5; MEKK5; M3K5_HUMAN. |
反应物种(已验证) |
Human,Mouse,Rat |
产品应用(已验证) |
WB,FCM |
产品应用(可推荐) |
ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1μg /test, |
克隆类型 |
多克隆 |
抗体来源 |
Rabbit |
理论分子量 |
155 |
细胞定位 |
细胞浆 |
性状 |
Liquid |
免疫原 |
KLH conjugated Synthesised phosphopeptide derived from human ASK1 around the phosphorylation site of Thr845 |
抗原表位 |
TE(p-T)FT |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
Cytoplasm. Endoplasmic reticulum. Note=Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region. |
Tissue |
Abundantly expressed in heart and pancreas. |
SIMILARITY |
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. |
SUBUNIT |
Homodimer when inactive. Binds both upstream activators and downstream substrates in multimolecular complexes. Associates with and inhibited by HIV-1 Nef. Part of a cytoplasmic complex made of HIPK1, DAB2IP and MAP3K5 in response to TNF. This complex form |
Function |
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fa |
Post-translational |
Phosphorylated at Thr-838 through autophosphorylation and by MAP3K6/ASK2 which leads to activation. Thr-838 is dephosphorylated by PPP5C. Ser-83 and Ser-1033 are inactivating phosphorylation sites, the former of which is phosphorylated by AKT1 and AKT2. P |
SWISS |
O35099 |
Gene ID |
4217 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. |