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货号 | bs-1731R-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Rabbit Anti-phospho-RAF1 (Ser338/Tyr340) antibody |
中文名称 | Rabbit Anti-phospho-RAF1 (Ser338/Tyr340) antibody |
产品标签 | 磷酸化抗体 |
研究领域 | 肿瘤,细胞生物,染色质和核信号,神经生物学,信号转导,细胞凋亡,细胞周期蛋白,转录调节因子,激酶和磷酸酶,细胞分化,线抗体, |
英文别名 | p-Raf-1(Ser338/Tyr340);RAF1; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); v-raf-1 murine leukemia viral oncogene homolog 1; cRaf; C-RAF; proto-oncogene c-RAF; 6430402F14Rik; AA990557; BB129353; c-Raf; Craf1; D830050J10Rik; MGC102375; Raf |
反应物种(已验证) | Human,Mouse |
反应物种(预测) | Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,GuineaPig |
产品应用(已验证) | WB,FCM |
产品应用(可推荐) | ELISA |
推荐稀释比例 | WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=2ug/Test, |
克隆类型 | 多克隆 |
抗体来源 | Rabbit |
理论分子量 | 73 |
细胞定位 | 细胞核,细胞浆,细胞膜 |
性状 | Liquid |
免疫原 | KLH conjugated Synthesised phosphopeptide derived from human RAF1 around the phosphorylation site of Ser338/Tyr340 |
抗原表位 | RD(p-S)S(p-Y)YW |
亚型 | IgG |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Note=Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphoryla |
Tissue | In skeletal muscle, isoform 1 is more abundant than isoform 2. [PTM] Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269, Ser-338, Tyr-341, Thr-491 and Ser-494 results in its activation. Phosphorylation at Ser-29, Ser-43, Se |
SIMILARITY | Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 RBD (Ras-binding) domain. |
SUBUNIT | Monomer. Homodimer. Heterodimerizes with BRAF and this heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activati |
Function | Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, different |
DISEASE | Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. I |
SWISS | P04049 |
Gene ID | 5894 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus. |