货号 |
bs-3140R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-Phospho-FoxO3a (Ser253) antibody |
中文名称 |
Rabbit Anti-Phospho-FoxO3a (Ser253) antibody |
产品标签 |
磷酸化抗体 |
研究领域 |
肿瘤,免疫学,染色质和核信号,神经生物学,信号转导,转录调节因子,表观遗传学, |
英文别名 |
FOXO3A (phospho S253); p=FOXO3A (phospho S253); FOXO1A (phospho S253); p-FOXO1A (phospho S253); AF6q21; AF6q21 protein; DKFZp781A0677; FKHR2; FKHRL 1; FKHRL1; FKHRL1P2; Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1; Forkhead box O3; Forkhead box |
反应物种(已验证) |
Human,Mouse,Rat |
反应物种(预测) |
Chicken,Dog,Pig,Cow,Horse,Sheep |
产品应用(已验证) |
IHC,FCM |
产品应用(可推荐) |
WB,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
克隆类型 |
多克隆 |
抗体来源 |
Rabbit |
理论分子量 |
71 |
细胞定位 |
细胞核,细胞浆 |
性状 |
Liquid |
免疫原 |
KLH conjugated Synthesised phosphopeptide derived from human FoxO3a around the phosphorylation site of Ser253 |
抗原表位 |
AV(p-S)MD |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
Cytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus upon oxidative stress and in the absence of survival factors. |
Tissue |
Ubiquitous. |
SIMILARITY |
Contains 1 fork-head DNA-binding domain. |
SUBUNIT |
Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration. Upon oxidative stress, interacts with STK4/MST1, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation. Interacts with P |
Function |
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MY |
Post-translational |
In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and pr |
DISEASE |
Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. |
SWISS |
O43524 |
Gene ID |
2309 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |