货号 |
bs-3232R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-phospho-IKK beta (Tyr199) antibody |
中文名称 |
Rabbit Anti-phospho-IKK beta (Tyr199) antibody |
产品标签 |
磷酸化抗体 |
研究领域 |
肿瘤,免疫学,信号转导,转录调节因子,激酶和磷酸酶,细胞粘附分子, |
英文别名 |
IKK beta (phospho Y199); IKK beta (phospho Tyr199); p-IKK beta (Tyr199); IKKI kappa B kinase 2; IKKβ; IKK β; I kappa B kinase beta; IkBKB; IKK 2; IKK B; IKK2; IKKB; Inhibitor of kappa light chain gene enhancer in B cells; Inhibitor of kappa light polypept |
反应物种(已验证) |
Human,Rat |
反应物种(预测) |
Mouse,Dog,Pig,Horse,Rabbit |
产品应用(已验证) |
WB,IHC |
产品应用(可推荐) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
克隆类型 |
多克隆 |
抗体来源 |
Rabbit |
理论分子量 |
87 |
细胞定位 |
细胞核,细胞浆,细胞膜 |
性状 |
Liquid |
免疫原 |
KLH conjugated Synthesised phosphopeptide derived from human IKK beta around the phosphorylation site of Tyr199 |
抗原表位 |
QK(p-Y)TV |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
Cytoplasm. Nucleus. Membrane raft. Note=Colocalized with DPP4 in membrane rafts. |
Tissue |
Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood. |
SIMILARITY |
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily. Contains 1 protein kinase domain. |
SUBUNIT |
Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins |
Function |
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK co |
Post-translational |
Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and PRKCZ; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents pro |
SWISS |
O14920 |
Gene ID |
3551 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
IKK beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK1. |