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货号 | bs-1643R-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Rabbit Anti-Phospho-Estrogen Receptor alpha (Tyr537) antibody |
中文名称 | Rabbit Anti-Phospho-Estrogen Receptor alpha (Tyr537) antibody |
产品标签 | 磷酸化抗体 |
研究领域 | 肿瘤,免疫学,染色质和核信号,神经生物学,信号转导,表观遗传学, |
英文别名 | Estrogen Receptor alpha (phospho Y537); p-Estrogen Receptor alpha (phospho Y537); ER alpha (Phospho-Tyr537); ER alpha (Phospho-Y537); Phospho-ER alpha (Y537); Phospho-ER alpha (Tyr537); p-ER alpha (Tyr537); p-ER alpha (Y537); Phospho-Estrogen Receptor alp |
反应物种(已验证) | Human |
反应物种(预测) | Mouse,Rat,Dog,Cow |
产品应用(已验证) | ICC,FCM |
产品应用(可推荐) | WB,IHC,IF,ELISA |
推荐稀释比例 | WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=0.2μg /test,IF=1:100-500,ICC=1:100, |
克隆类型 | 多克隆 |
抗体来源 | Rabbit |
理论分子量 | 66 |
细胞定位 | 细胞核,细胞浆,细胞膜 |
性状 | Liquid |
免疫原 | KLH conjugated Synthesised phosphopeptide derived from human ER alpha around the phosphorylation site of Tyr537 |
抗原表位 | PL(p-Y)DL |
亚型 | IgG |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Isoform 1: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=A minor fraction is associated with the inner membrane. Isoform 3: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell |
Tissue | Widely expressed. Isoform 3 is not expressed in the pituitary gland. |
SIMILARITY | Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain. |
SUBUNIT | Binds DNA as a homodimer. Can form a heterodimer with ESR2. Isoform 3 can probably homodimerize or heterodimerize with isoform 1 and ESR2. Interacts with FOXC2, MAP1S, SLC30A9, UBE1C and NCOA3 coactivator (By similarity). Interacts with EP300; the interac |
Function | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves eith |
Post-translational | Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Self-association induces phosphorylation. Glycosylated; contains N-acetylglucosamine, probably O-linked. Ubiquitinated. Deubiquitinated by OTUB1. |
SWISS | P03372 |
Gene ID | 2099 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | Estrogen and progesterone receptor are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboxy terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ER Alpha and ER Beta. ER Alpha and ER Beta have been shown to be differentially activated by various ligands. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-A and hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors. |