货号 |
bs-5216R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-Phospho-Bad (Ser118) antibody |
中文名称 |
Rabbit Anti-Phospho-Bad (Ser118) antibody |
产品标签 |
磷酸化抗体 |
研究领域 |
肿瘤,细胞生物,免疫学,神经生物学,信号转导,细胞凋亡,线抗体, |
英文别名 |
Bad (phospho S118); Bad (phospho Ser118); p-Bad (S118);p- Bad (Ser118); p-Bad (phospho Ser118); BBC 2; BBC2; BBC6; Bcl 2 Antagonist of Cell Death; Bcl 2 Binding Component 6; BCL X / BCL 2 Binding Protein; BCL X Binding Protein; Bcl XL/Bcl 2 Associated Dea |
反应物种(已验证) |
Human,Mouse,Rat |
反应物种(预测) |
Dog,Pig,Cow,Horse,Rabbit |
产品应用(已验证) |
WB,IHC,FCM |
产品应用(可推荐) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500, |
克隆类型 |
多克隆 |
抗体来源 |
Rabbit |
理论分子量 |
22 |
细胞定位 |
细胞浆,细胞膜 |
性状 |
Liquid |
免疫原 |
KLH conjugated Synthesised phosphopeptide derived from human BAD around the phosphorylation site of Ser118 |
抗原表位 |
RM(p-S)DE |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein G |
SUBCELLULAR |
Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm. |
Tissue |
Expressed in a wide variety of tissues. |
SIMILARITY |
Belongs to the Bcl-2 family. |
SUBUNIT |
Forms heterodimers with the anti-apoptotic proteins, Bcl-X(L), Bcl-2 and Bcl-W. Also binds protein S100A10. The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins. Interacts with AKT1 and PIM3. |
Function |
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to |
Post-translational |
Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 andSer-134 in response to survival stimuli, which blocks itspro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75promotes heterodimerization with 14-3-3 proteins. This interactionthen facilita |
SWISS |
Q92934 |
Gene ID |
572 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform. |