货号 |
bs-10441R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) antibody |
中文名称 |
Rabbit Anti-phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) antibody |
产品标签 |
磷酸化抗体 |
研究领域 |
细胞生物,信号转导,细胞凋亡,激酶和磷酸酶 |
英文别名 |
phospho-MST4+MST3+STK25(phospho T178 + T190 + T174);
p-MST4 + MST3 + STK25 (phospho T178 + T190 + T174);
Mammalian STE20 like protein kinase 3; Mammalian Ste20 like protein kinase 4; Mammalian sterile 20 like 4; MASK; mess1; MGC94619; MST 3; MST 4; MST; M |
反应物种(已验证) |
Human,Mouse,Rat |
反应物种(预测) |
Cow,Horse,Sheep |
产品应用(已验证) |
WB,IHC |
产品应用(可推荐) |
ICC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
克隆类型 |
多克隆 |
抗体来源 |
Rabbit |
理论分子量 |
48 |
细胞定位 |
细胞核,细胞浆,细胞膜 |
性状 |
Liquid |
免疫原 |
KLH conjugated synthesised phosphopeptide derived from human MST4/MST3/STK25 around the phosphorylation site of Thr178/Thr190/Thr174 |
抗原表位 |
RN(p-T)FV |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
STK25, MST4: Cytoplasmic, Golgi Apparatus. MST3: Cytoplasm. Nucleus. Membrane. Note=The truncated form (MST3/N) translocates to the nucleus. Co-localizes with STK38L in the membrane. |
Tissue |
MST3: Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex. |
SIMILARITY |
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain. |
SUBUNIT |
Monomer. |
Function |
Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and |
Post-translational |
MST3: Proteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis.
Isoform B is activated by phosphorylation by PKA. Oxi |
SWISS |
O00506 |
Gene ID |
10494 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
Novel human Ste20-related kinase Mst4 is biologically active in the activation of MEK/ERK pathway and in mediating cell growth and transformation. It is pro apoptotic and is highly expressed in placenta, thymus, and peripheral blood leukocytes. Interaction with Golgi matrix protein GOLGA2 results in autophosphorylation on Thr-178, possibly as a consequence of stabilization of dimer formation. This may also be activated by C terminal cleavage. MST3 or Mammalian Sterile 20-like kinase 3 is a member of the germinal center kinase-III family. MST3 contains a conserved kinase domain at its NH(2)-terminus and a regulatory domain at its COOH-terminus. Caspase-mediated cleavage of the regulatory domain of MST3 activates its intrinsic kinase activity and leads to nuclear translocation. Expression of COOH-terminal truncated MST3 in cells results in DNA fragmentation and induction of apoptosis. It can inhibit cell migration in a fashion dependent on autophosphorylation and can regulate paxillin phosphorylation through tyrosine phosphatase PTP-PEST. Mitogen activated protein kinase cascades have been conserved throughout evolution. In mammals, these cascades allow responses to complex stimuli such as growth factors and inflammatory cytokines. In yeast, STK25 functions upstream of the MAPK cascade. |