货号 |
bs-12412R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-phospho-SP1 (Thr453) antibody |
中文名称 |
Rabbit Anti-phospho-SP1 (Thr453) antibody |
产品标签 |
磷酸化抗体 |
研究领域 |
肿瘤,细胞生物,发育生物学,神经生物学,信号转导,干细胞,转录调节因子,锌指蛋白,表观遗传学, |
英文别名 |
SP1 (phospho T453); p-SP1 (phospho T453); SP1 (phospho-Thr453); SP1 (phospho-T453); p-SP1 (phospho T453); p-TSFP1 (phospho T453); Sp1 transcription factor isoform a; TSFP1; TSFP 1; Specificity protein 1; Transcription factor Sp1; SP1_HUMAN. |
反应物种(已验证) |
Human |
反应物种(预测) |
Mouse,Rat |
产品应用(已验证) |
IHC |
产品应用(可推荐) |
WB,ICC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100, |
克隆类型 |
多克隆 |
抗体来源 |
Rabbit |
理论分子量 |
81 |
细胞定位 |
细胞核,细胞浆 |
性状 |
Liquid |
免疫原 |
KLH conjugated synthesised phosphopeptide derived from human SP1 around the phosphorylation site of Thr453 |
抗原表位 |
IR(p-T)PT |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location. |
Tissue |
Up-regulated in adenocarcinomas of the stomach (at protein level). |
SIMILARITY |
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. |
SUBUNIT |
Interacts with ATF7IP, ATF7IP2, BAHD1, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein. Interacts with HIV-1 Vpr; the interaction is inhibited by SP1 O-glycosylation. Interacts with SV40 VP2/3 proteins. Interacts with SV40 m |
Function |
Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes su |
Post-translational |
Phosphorylated on multiple serine and threonine residues. Phosphorylation is coupled to ubiquitination, sumoylation and proteolytic processing. Phosphorylation on Ser-59 enhances proteolytic cleavage. Phosphorylation on Ser-7 enhances ubiquitination and p |
SWISS |
P08047 |
Gene ID |
6667 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21-bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A) (7), may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.1. |