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| 货号 | bs-3770R-1 |
| 品牌 | |
| 浓度 | |
| 货期 | 现货 |
| 英文名称 | Rabbit Anti-Histone H3 (Di Methyl K4) antibody |
| 中文名称 | Rabbit Anti-Histone H3 (Di Methyl K4) antibody |
| 产品标签 | 甲基化抗体 |
| 研究领域 | 肿瘤,细胞生物,免疫学,染色质和核信号,转录调节因子,表观遗传学, |
| 英文别名 | Histone H3(Di Methyl Lys4); H3K4me2; Di methyl Histone H3(Lys4); Di methyl Histone H3(K4); H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H |
| 反应物种（已验证） | Human,Mouse,Rat |
| 反应物种（预测） | Pig,Cow,Rabbit,Fruit Fly |
| 产品应用（已验证） | WB,IHC,FCM |
| 产品应用（可推荐） | ICC,IF,ELISA |
| 推荐稀释比例 | WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=0.2μg /Test,IF=1:100-500,ICC=1:100-500, |
| 克隆类型 | 多克隆 |
| 抗体来源 | Rabbit |
| 理论分子量 | 15 |
| 细胞定位 | 细胞核 |
| 性状 | Liquid |
| 免疫原 | KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of Di Methyl K4 |
| 抗原表位 | RT(Di Methyl-K)QT |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| SUBCELLULAR | Nucleus. Chromosome. Note=Localizes to both the large, transcriptionally active, somatic macronucleus (MAC) and the small, transcriptionally inert, germ line micronucleus (MIC). |
| SIMILARITY | Belongs to the histone H3 family. |
| SUBUNIT | The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with GCN5, whereby H3S10ph increases hist |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replic |
| Post-translational | Phosphorylated to form H3S10ph. H3S10ph promotes subsequent H3K14ac formation by GCN5. H3S10ph is only found in the mitotically dividing MIC, but not in the amitotically dividing MAC. H3S10ph is correlated with chromosome condensation during mitotic or me |
| SWISS | P68431 |
| Gene ID | 8350 |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 英文介绍 | Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. |
