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货号 | bs-13084R-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Rabbit Anti-phospho-Ephexin-1 (Tyr179) antibody |
中文名称 | Rabbit Anti-phospho-Ephexin-1 (Tyr179) antibody |
产品标签 | 磷酸化抗体 |
研究领域 | 细胞生物,神经生物学,信号转导,G蛋白偶联受体,G蛋白信号, |
英文别名 | Ephexin-1 (phospho Y179); p-Ephexin-1 (phospho Y179); ARHGEF27; Eph-interacting exchange protein; EPHEXIN; Ephexin 1; Neuronal guanine nucleotide exchange factor; Ngef; NGEF_HUMAN. |
反应物种(已验证) | Human,Mouse,Rat |
反应物种(预测) | Chicken |
产品应用(已验证) | WB |
产品应用(可推荐) | ELISA |
推荐稀释比例 | WB=1:500-2000,Elisa=1:5000-10000, |
克隆类型 | 多克隆 |
抗体来源 | Rabbit |
理论分子量 | 82 |
细胞定位 | 细胞浆,细胞膜 |
性状 | Liquid |
免疫原 | KLH conjugated synthesised phosphopeptide derived from human Ephexin-1 around the phosphorylation site of Tyr179 |
抗原表位 | LL(p-Y)QE |
亚型 | IgG |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Cytoplasm. Membrane. Cell projection, growth cone. Associated with membranes. Localizes to axonal growth cones. |
Tissue | Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung. |
SIMILARITY | Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. Contains 1 SH3 domain. Database links : UniProtKB/Swiss-Prot: Q8N5V2.2 |
SUBUNIT | Interacts with CDK5R1 and EPHA4; activated by EPHA4 through the CDK5 kinase. |
Function | Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity swi |
Post-translational | Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA. |
SWISS | Q8N5V2 |
Gene ID | 25791 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | EPHEXIN is a 710 amino acid protein that localizes to both the membrane and the cytoplasm and contains one SH3 domain, one PH domain and one DH domain. Expressed at high levels in brain and present at lower levels in lung tissue, EPHEXIN interacts with EphA4 and functions as a guanine nucleotide exchange factor (GEF) that is capable of activating Rho A, Rac 1 and Cdc42 and is thought to play a role in axon guidance and growth cone collapse. EPHEXIN is subject to Src-dependent phosphorylation, an event that increases the GEF activity of EPHEXIN toward Rho A. Human EPHEXIN, which exists as multiple alternatively spliced isoforms, shares a high degree of sequence homology with its mouse counterpart, suggesting a conserved role between species. |