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货号 | bs-11961R-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Rabbit Anti-SNAIL + SLUG antibody |
中文名称 | Rabbit Anti-SNAIL + SLUG antibody |
研究领域 | 肿瘤,细胞生物,免疫学,神经生物学,信号转导,干细胞,转录调节因子,表观遗传学, |
英文别名 | SNAIL+SLUG; dJ710H13.1; Protein sna; Protein snail homolog; SLUGH2; SNA; Sna protein; SNAH; SNAI1; Snail homolog 1 (Drosophila); Zinc finger protein SNAI1; SNAI1_HUMAN; Protein snail homolog 1; dJ710H13.1; SNAIL; SNAIL1; Protein sna; Neural crest transcri |
反应物种(已验证) | Human,Mouse,Rat |
反应物种(预测) | Chicken,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig,Danio rerio |
产品应用(已验证) | WB,FCM |
产品应用(可推荐) | IHC,IF,ELISA |
推荐稀释比例 | WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500, |
克隆类型 | 多克隆 |
抗体来源 | Rabbit |
理论分子量 | 30 |
细胞定位 | 细胞核,细胞浆 |
性状 | Liquid |
免疫原 | KLH conjugated synthetic peptide derived from human SNAIL + SLUG |
抗原表位 | 151-250/264 |
亚型 | IgG |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Nucleus. Cytoplasm. Note=Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. |
Tissue | Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. |
SIMILARITY | Belongs to the snail C2H2-type zinc-finger protein family. Contains 4 C2H2-type zinc fingers. |
SUBUNIT | Interacts with FBXL14 and GSK3B. Interacts with BTRC; interaction occurs when it is phosphorylated on the destruction motif. Interacts (via SNAG domain) with WTIP (via LIM domains). Interacts (via SNAG domain) with LIMD1 (via LIM domains), and AJUBA (via |
Function | Involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm. Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. |
Post-translational | Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phos |
SWISS | O43623 |
Gene ID | 6615 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | SNAIL: The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]. SLUG: This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008]. |