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货号 | bs-3266R-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Rabbit Anti-Phospho-MDM2 (Ser166) antibody |
中文名称 | Rabbit Anti-Phospho-MDM2 (Ser166) antibody |
产品标签 | 磷酸化抗体 |
研究领域 | 肿瘤,细胞生物,细胞凋亡,细胞周期蛋白,转录调节因子,表观遗传学, |
英文别名 | MDM2 (phospho S166); p-MDM2 (phospho S166); MDM2(Phospho Ser166); MDM2 (phospho S166); Double minute 2 protein; Hdm 2; HDM2; MDM 2; Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa; MDM2BP; Mouse Double Minute 2 |
反应物种(已验证) | Human |
反应物种(预测) | Mouse,Rat,Horse,Rabbit |
产品应用(已验证) | IHC,ICC,FCM |
产品应用(可推荐) | IF |
推荐稀释比例 | IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500,ICC=1:100, |
克隆类型 | 多克隆 |
抗体来源 | Rabbit |
理论分子量 | 55 |
细胞定位 | 细胞核,细胞浆 |
性状 | Liquid |
免疫原 | KLH conjugated synthesised phosphopeptide derived from human MDM2 around the phosphorylation site of Ser166 |
抗原表位 | AI(p-S)ET |
亚型 | IgG |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Note=Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may |
Tissue | Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues. |
SIMILARITY | Belongs to the MDM2/MDM4 family. Contains 1 RanBP2-type zinc finger. Contains 1 RING-type zinc finger. Contains 1 SWIB domain. |
SUBUNIT | Binds p53/TP53, TP73/p73, ARF/P14, PML, RBL5 and RP11, and specifically to RNA. Can interact with RB1, E1A-associated protein EP300 and the E2F1 transcription factor. Forms a ternary complex with p53/TP53 and WWOX. Interacts with CDKN2AIP, MTBP, RFWD3, TB |
Function | E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an |
Post-translational | Phosphorylated in response to ionizing radiation in an ATM-dependent manner. Phosphorylation on Ser-166 by SGK1 activates ubiquitination of p53/TP53.
Auto-ubiquitinated; which leads to proteasomal degradation. Also ubiquitinated by TRIM13. Deubiquitin |
DISEASE | Note=Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Fou |
SWISS | Q00987 |
Gene ID | 4193 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | Inhibits TP53/p53- and TP73/p73-mediated cell cyclearrest and apoptosis by binding its transcriptional activation domain. Functions as a ubiquitin ligase E3, in the presence of E1 and E2, toward p53 and itself. Permits the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Binds p53, p73, ARF(P14), ribosomal protein L5 and specifically to RNA. Can interact also with retinoblastoma protein(RB), E1A-associated protein EP300 and the E2F1 transcription factor. Forms a ternary complex with TP53/p53 and WWOX. Interacts with CDKN2AIP, MTBP, TRBG1 and USP7. Isoform Mdm2-F does not interact with TP53/p53. Interacts with PYHIN1. Interacts with, and ubiquitinates HIV-1 Tat. Belongs to the MDM2/MDM4 family. |