货号 |
bs-33017R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-S-tag antibody |
中文名称 |
Rabbit Anti-S-tag antibody |
产品标签 |
标签抗体 |
研究领域 |
细胞生物 |
英文别名 |
Stag; S tag; Ribonuclease A; KETAAAKFERQHMDS. |
反应物种(预测) |
Species independent |
产品应用(已验证) |
WB |
产品应用(可推荐) |
ELISA |
推荐稀释比例 |
WB=1:1000-5000,Elisa=1:5000-10000, |
克隆类型 |
多克隆 |
抗体来源 |
Rabbit |
性状 |
Liquid |
免疫原 |
KLH conjugated synthetic peptide S-tag |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag.
The amino acid sequence of the S-tag is: Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser. It is believed that the peptide with its abundance of charged and polar residues could improve solubility of proteins it is attached to[citation needed]. Moreover, the peptide alone is thought not to fold into a distinct structure. On DNA-level the S-tag can be attached to the N- or C-terminus of any protein. After gene expression, such a tagged protein can be detected by commercially available antibodies. |