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货号 | bsm-52225R-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Rabbit Anti-Smad4 antibody |
中文名称 | Rabbit Anti-Smad4 antibody |
产品标签 | 重组兔单抗 |
研究领域 | 肿瘤,细胞生物,信号转导,细胞凋亡,生长因子和激素 |
英文别名 | Mothers against decapentaplegic homolog 4; (Small) Mothers Against Decapentaplegic; Deleted in Pancreatic Carcinoma; Deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; DPC 4; DPC4; hSMAD4; JIP; MAD homolog 4; MAD mothers a |
反应物种(已验证) | Human,Mouse |
反应物种(预测) | Rat |
产品应用(已验证) | WB,FCM |
产品应用(可推荐) | IHC,IF |
推荐稀释比例 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
克隆类型 | 单克隆 |
克隆号 | 3A1 |
抗体来源 | Rabbit |
理论分子量 | 60 |
细胞定位 | 细胞核,细胞浆 |
性状 | Liquid |
免疫原 | Recombinant human Smad4 protein (1-200aa) |
亚型 | IgG |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R-SMAD. PDPK1 prevents its nuclear translocation in response to TGF-beta. |
SIMILARITY | Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain. Contains 1 MH2 (MAD homology 2) domain. |
SUBUNIT | Interacts with CITED2 (By similarity). Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the t |
Function | Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Prom |
Post-translational | Phosphorylated by PDPK1.
Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiqitinat |
DISEASE | Pancreatic cancer (PNCA) [MIM:260350]: A malignant neoplasm of the pancreas. Tumors can arise from both the exocrine and endocrine portions of the pancreas, but 95% of them develop from the exocrine portion, including the ductal epithelium, acinar cells, |
SWISS | Q13485 |
Gene ID | 4089 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | Smad 4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 4 is the common Smad (co-Smad). Previously identified as the tumor suppressor DPC4 (deleted in pancreatic carcinoma, locus 4), Smad 4 is functionally distinct among the Smad family, and is required for the assembly and transcriptional activation of diverse, Smad-DNA complexes. In contrast to the R-Smads, Smad 4 is not regulated by phosphorylation, but acts as a common mediator of TGF-Beta, activin, and bone morphogenetic protein signaling responses. Smad 4 is frequently inactivated in pancreatic, biliary and colorectal tumors. |