货号 |
bsm-52068R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-ERK2 antibody |
中文名称 |
Rabbit Anti-ERK2 antibody |
产品标签 |
重组兔单抗 |
研究领域 |
肿瘤,细胞生物,信号转导,转录调节因子,激酶和磷酸酶 |
英文别名 |
Mitogen-Activated Protein Kinase 1;
Extracellular Signal-Regulated Kinase 2;
Mitogen-Activated Protein Kinase 2;
MAP Kinase Isoform P42;
MAP Kinase 1;
MAP Kinase 2;
EC 2.7.11.24;
P42-MAPK;
MAPK 2;
PRKM1;
PRKM2;
ERK-2;
ERK2;
ERT1;
Protein Tyr |
反应物种(已验证) |
Human,Mouse,Rat |
产品应用(已验证) |
WB,IHC,FCM |
产品应用(可推荐) |
ICC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:50-200,IHC-F=1:50-200,Flow Cyt=1ug/Test,IF=1:50-200,ICC=1:100, |
克隆类型 |
单克隆 |
克隆号 |
3G1 |
抗体来源 |
Rabbit |
理论分子量 |
42 |
细胞定位 |
细胞核,细胞浆,细胞膜 |
性状 |
Liquid |
免疫原 |
Recombinant human ERK2 protein, around C-terminal 150aa |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
Cytoplasm, cytoskeleton, spindle. Nucleus. Cytoplasm, cytoskeleton, centrosome. Cytoplasm. Note=Associated with the spindle duringprometaphase and metaphase. PEA15-binding andphosphorylated DAPK1 promote its cytoplasmic retention.Phosphorylation at Ser-24 |
Tissue |
Widely expressed. |
SIMILARITY |
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. |
SUBUNIT |
Binds both upstream activators and downstream substrates in multimolecular complexes. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with ADAM15, ARHGEF2, ARRB2, DAPK1 (via death domain), HSF4, |
Function |
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initia |
Post-translational |
Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Undergoes regulatory phosphorylation on additional residues such as Ser-246 and Ser-248 in the kinase insert domain (KID) |
SWISS |
P28482 |
Gene ID |
5594 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014] |