货号 |
bsm-52223R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-Smad2 antibody |
中文名称 |
Rabbit Anti-Smad2 antibody |
产品标签 |
重组兔单抗 |
研究领域 |
肿瘤,细胞生物,信号转导,细胞凋亡,生长因子和激素,转录调节因子,激酶和磷酸酶 |
英文别名 |
Mothers against decapentaplegic homolog 2; SMAD 2; Mothers against DPP homolog 2; Smad2; hMAD 2; hSMAD2; JV18 1; JV18; JV181; MAD; MAD Related Protein 2; MADH2; MADR2; MGC22139; MGC34440; Mothers Against Decapentaplegic Homolog 2; mothers against DPP homo |
反应物种(已验证) |
Human,Mouse,Rat |
产品应用(已验证) |
WB,IHC,ICC,FCM |
产品应用(可推荐) |
IF |
推荐稀释比例 |
WB=1:500-1000,IHC-P=1:50-200,IHC-F=1:50-200,Flow Cyt=2ug/Test,IF=1:50-200,ICC=1:100, |
克隆类型 |
单克隆 |
克隆号 |
9A3 |
抗体来源 |
Rabbit |
理论分子量 |
52 |
细胞定位 |
细胞核,细胞浆 |
性状 |
Liquid |
免疫原 |
Recombinant human Smad2 protein, around N-terminal 1-200aa |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of |
Tissue |
Expressed at high levels in skeletal muscle, heart and placenta. |
SIMILARITY |
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. |
SUBUNIT |
Momomer; the absence of TGF-beta. Heterodimer; in the presence of TGF-beta. Forms a heterodimer with co-SMAD, SMAD4, in the nucleus to form the transactivation complex SMAD2/SMAD4. Interacts with AIP1, HGS, PML and WWP1. Interacts with NEDD4L in response |
Function |
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes th |
Post-translational |
Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting |
SWISS |
Q15796 |
Gene ID |
4087 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGF-Beta superfamily of modulators. Like many other TGF-Beta family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGF-Beta's. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase. This antibody is cross reactive with Smad3. |