货号 |
bsm-52134R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-phospho-ATF2 (Thr71) antibody |
中文名称 |
Rabbit Anti-phospho-ATF2 (Thr71) antibody |
产品标签 |
磷酸化抗体,重组兔单抗 |
研究领域 |
细胞生物,转录调节因子,表观遗传学, |
英文别名 |
ATF2(T71); ATF2 (phospho T71 + T53); phospho-ATF2(Thr53)(mo); ATF2 (phospho T71); p-ATF2 (phospho T71); ATF2 (phospho Thr71); p-ATF2 (phospho Thr71); CREB 2; HB 16;Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Bindin |
反应物种(已验证) |
Human,Mouse,Rat |
产品应用(已验证) |
WB,IHC |
产品应用(可推荐) |
ICC,IF |
推荐稀释比例 |
WB=1:500-1000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC=1:50-200, |
克隆类型 |
单克隆 |
克隆号 |
7B1 |
抗体来源 |
Rabbit |
理论分子量 |
54 |
细胞定位 |
细胞核 |
性状 |
Liquid |
免疫原 |
KLH conjugated synthesised phosphopeptide derived from human ATF2 around the phosphorylation site of Thr71 |
抗原表位 |
TP(p-T)PT |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
Nucleus. |
Tissue |
Abundant expression seen in the brain. |
SIMILARITY |
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. |
SUBUNIT |
Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. |
Function |
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferential |
Post-translational |
Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stres |
SWISS |
P15336 |
Gene ID |
1386 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain. |