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货号 | bsm-52187R-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Rabbit Anti-Phospho-PKC alpha (Thr638) antibody |
中文名称 | Rabbit Anti-Phospho-PKC alpha (Thr638) antibody |
产品标签 | 磷酸化抗体,重组兔单抗 |
研究领域 | 肿瘤,免疫学,信号转导,细胞凋亡,转录调节因子,激酶和磷酸酶 |
英文别名 | PKC alpha(T638); PKC(Phospho-Thr641); Phospho-PKC alpha/beta II (Thr638/641); Phospho-PKC alpha/beta II (Thr638 + 641); PKC alpha; AAG6; Aging associated gene 6; PICK 1; PKC A; PKC alpha; PKCA; PRKACA; PRKC A; PRKCA; Protein Kinase C alpha; Protein kinase |
反应物种(已验证) | Human,Mouse,Rat |
反应物种(预测) | Chicken,Pig,Cow,Horse |
产品应用(已验证) | WB,IHC,ICC,FCM |
产品应用(可推荐) | IF |
推荐稀释比例 | WB=1:500-2000,IHC-P=1:50-1:500,IHC-F=1:50-1:500,Flow Cyt=2ug/Test,IF=1:50-1:500,ICC=1:50, |
克隆类型 | 单克隆 |
克隆号 | 4B3 |
抗体来源 | Rabbit |
理论分子量 | 77 |
细胞定位 | 细胞核,细胞浆,细胞膜 |
性状 | Liquid |
免疫原 | KLH conjugated synthesised phosphopeptide derived from human PKC alpha around the phosphorylation site of Thr638 |
抗原表位 | VL(p-T)PP |
亚型 | IgG |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Cytoplasm. Cell membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein (Probable). Nucleus. |
SIMILARITY | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain. |
SUBUNIT | Recruited in a circadian manner into a nuclear complex which also includes BMAL1 and GNB2L1/RACK1 (By similarity). Interacts with ADAP1/CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine. Interacts with PICK1 (via PDZ domain). |
Function | Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-d |
Post-translational | Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. A |
SWISS | P17252 |
Gene ID | 5578 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation. |