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货号 | bsm-52208R-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Rabbit Anti-phospho-Src (Tyr419) antibody |
中文名称 | Rabbit Anti-phospho-Src (Tyr419) antibody |
产品标签 | 磷酸化抗体,重组兔单抗 |
研究领域 | 肿瘤,细胞生物,发育生物学,染色质和核信号,信号转导,转录调节因子,激酶和磷酸酶,线抗体,表观遗传学, |
英文别名 | Src(Y419); Src (phospho Y419); p-Src (phospho Y419); ASV; Avian sarcoma virus; c SRC; CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA; cSrc; EC 2.7.10.2; Neuronal CSRC tyrosine specific prot |
反应物种(已验证) | Human |
反应物种(预测) | Mouse,Rat |
产品应用(已验证) | WB,FCM |
产品应用(可推荐) | IHC,ICC,IF |
推荐稀释比例 | WB=1:500-1000,IHC-P=1:50-100,IHC-F=1:50-100,Flow Cyt=1:50-100,IF=1:50-100,ICC=1:50-100, |
克隆类型 | 单克隆 |
克隆号 | 23B1 |
抗体来源 | Rabbit |
理论分子量 | 60 |
细胞定位 | 细胞核,细胞浆,细胞膜 |
性状 | Liquid |
免疫原 | KLH conjugated Synthesised phosphopeptide derived from human Src around the phosphorylation site of Tyr419 |
抗原表位 | NE(p-Y)TA |
亚型 | IgG |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Cell membrane. Mitochondrion inner membrane. Nucleus. Cytoplasm, cytoskeleton. Note=Localizes to focal adhesion sites after integrin engagement. Localization to focal adhesion sites requires myristoylation and the SH3 domain. |
Tissue | Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues. |
SIMILARITY | Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain. |
SUBUNIT | Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1. Interacts with the cytoplasmic domain of MUC1, phosphorylates it and increases binding of MUC1 with beta-catenin. Interacts with RALGPS1 |
Function | Non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled |
Post-translational | Myristoylated at Gly-2, and this is essential for targeting to membranes.
Dephosphorylated at Tyr-535 by PTPRJ. Phosphorylated on Tyr-535 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-424. Normall |
DISEASE | Note=SRC kinase activity has been shown to be increased in several tumor tissues and tumor cell lines such as colon carcinoma cells. |
SWISS | P12931 |
Gene ID | 6714 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |