货号 |
bsm-52566R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-Caspase-9 antibody |
中文名称 |
Rabbit Anti-Caspase-9 antibody |
产品标签 |
重组兔单抗 |
研究领域 |
肿瘤,细胞生物,细胞凋亡 |
英文别名 |
Caspase-9 subunit p35; Apaf-3; APAF 3; APAF3; Apoptosis related cysteine peptidase; Apoptotic protease activating factor 3; Apoptotic protease MCH 6; Apoptotic protease MCH6; CASP 9; CASP9; Caspase 9; Caspase 9 apoptosis related cysteine protease; Caspase |
中文别名 |
活化半胱胺酸蛋白酶蛋白-9抗体 |
反应物种(已验证) |
Human |
反应物种(预测) |
Mouse |
产品应用(已验证) |
WB |
产品应用(可推荐) |
IP |
推荐稀释比例 |
WB=1:1000-2000,IP=1:10-50, |
克隆类型 |
单克隆 |
克隆号 |
1D7 |
抗体来源 |
Rabbit |
理论分子量 |
35/50 |
细胞定位 |
细胞浆 |
性状 |
Liquid |
免疫原 |
KLH conjugated synthetic peptide derived from human Caspase-9 |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
Tissue |
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. |
SIMILARITY |
Belongs to the peptidase C14A family.
Contains 1 CARD domain. |
SUBUNIT |
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cyt |
Function |
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).< |
Post-translational |
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to bl |
SWISS |
P55211 |
Gene ID |
842 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, and following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 intereacts with BIRC7 and has been shown to cleave PARP and vimentin. |