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货号 | bsm-41634M-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Mouse Anti-SARS-CoV-2 (2019-nCoV) Spike Neutralizing antibody |
中文名称 | Mouse Anti-SARS-CoV-2 (2019-nCoV) Spike Neutralizing antibody |
研究领域 | 细菌及病毒, |
英文别名 | SARS-CoV-2 spike protein; 2019-nCOV Spike protein; Spike glycoprotein; Spike protein S1; Surface glycoprotein; Spike protein RBD; SPIKE_SARS2. |
反应物种(预测) | SARS-CoV-2 |
产品应用(可推荐) | ELISA |
推荐稀释比例 | Elisa=1:5000-10000, |
克隆类型 | 单克隆 |
克隆号 | 7G10 |
抗体来源 | Mouse |
理论分子量 | 140 |
性状 | Liquid |
免疫原 | Recombinant SARS-CoV-2 Spike S1 Protein |
抗原表位 | 14-685/1213 |
亚型 | IgG1 |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Virion membrane ; Single-pass type I membrane protein ; Host endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein ; Host cell membrane ; Single-pass type I membrane protein ; Note: Accumulates in the endop |
Tissue | The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes. Specific enzymatic cleavages in vivo yield mature proteins. The precurssor is processed into S1 and S2 by host cell furin or another cellular protea |
SUBUNIT | Spike glycoprotein: Homotrimer; each monomer consists of a S1 and a S2 subunit (PubMed:32155444, PubMed:32075877). The resulting peplomers protrude from the virus surface as spikes (By similarity). Interacts with the accessory proteins 3a and 7a.< |
Function | attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the |
SWISS | P0DTC2 |
Gene ID | 43740568 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |