货号 |
bs-0741R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-CDKN1A/p21 antibody |
中文名称 |
Rabbit Anti-CDKN1A/p21 antibody |
研究领域 |
肿瘤,细胞生物,信号转导,细胞凋亡,细胞周期蛋白 |
英文别名 |
CDN1A_HUMAN; Cyclin-dependent kinase inhibitor 1; CDKN1A; CAP20; CDKN1; CIP1; MDA6; MDA-6; PIC1; SDI1; WAF1; p21CIP1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6 (MDA-6); |
反应物种(已验证) |
Human,Mouse,Rat |
反应物种(预测) |
Chicken,Dog,Cow |
产品应用(已验证) |
IHC,ICC,FCM |
产品应用(可推荐) |
IF,ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test ,IF=1:100-500,ICC=1:100, |
克隆类型 |
多克隆 |
抗体来源 |
Rabbit |
理论分子量 |
18 |
细胞定位 |
细胞核,细胞浆 |
性状 |
Liquid |
免疫原 |
KLH conjugated synthetic peptide derived from human P21 |
抗原表位 |
101-164/164 |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
Cytoplasmic and Nuclear. |
Tissue |
Expressed in all adult tissues, with 5-fold lower levels observed in the brain. |
SIMILARITY |
Belongs to the CDI family. |
SUBUNIT |
Interacts with HDAC1; the interaction is prevented by competitive binding of C10orf90/FATS to HDAC1 facilitating acetylation and protein stabilization of CDKN1A/p21. Interacts with MKRN1. Interacts with PSMA3. Interacts with PCNA. Component of the ternary |
Function |
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kina |
Post-translational |
Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell prolife |
SWISS |
P39689 |
Gene ID |
1026 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015] |