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货号 | bs-0301R-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Rabbit Anti-Dnmt3b antibody |
中文名称 | Rabbit Anti-Dnmt3b antibody |
研究领域 | 肿瘤,细胞生物,信号转导,表观遗传学, |
英文别名 | Cytosine 5methyltransferase 3B; DNA (cytosine 5) methyltransferase 3 beta; DNA; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; Dnmt3b; Dnmt3 Beta; EC 2.1.1.37; ICF; M.HsaIIIB; MGC124407; RP23-89H14.3; DNM3B_HUMAN. |
反应物种(已验证) | Human,Mouse |
反应物种(预测) | Rat |
产品应用(已验证) | IHC,ICC,FCM |
产品应用(可推荐) | ELISA |
推荐稀释比例 | Elisa=1:5000-10000,IHC-P=1:100-500,Flow Cyt=1μg/Test,ICC=1:100, |
克隆类型 | 多克隆 |
抗体来源 | Rabbit |
理论分子量 | 94 |
细胞定位 | 细胞核 |
性状 | Liquid |
免疫原 | KLH conjugated synthetic peptide derived from human Dnmt3 Beta |
抗原表位 | 1-80/853 |
亚型 | IgG |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Nucleus. |
Tissue | Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, s |
SIMILARITY | Belongs to the C5-methyltransferase family. Contains 1 ADD domain. Contains 1 GATA-type zinc finger. Contains 1 PHD-type zinc finger. Contains 1 PWWP domain. |
SUBUNIT | Interacts with BAZ2A/TIP5, SUV39H1 and CBX4. Interacts with DNMT1 and DNMT3A, SETDB1, UBL1, UBE2I9 and ZHX1. Interacts with the PRC2/EED-EZH2 complex. |
Function | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucle |
Post-translational | Sumoylated. |
DISEASE | Defects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome type 1 (ICF1) [MIM:242860]. A rare disorder characterized by a variable immunodeficiency, facial anomalies, and branching of chromosomes 1, 9, and 16. Othe |
SWISS | Q9UBC3 |
Gene ID | 1789 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome. |