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货号 | bs-0497R-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Rabbit Anti-Dnmt3a antibody |
中文名称 | Rabbit Anti-Dnmt3a antibody |
研究领域 | 肿瘤,细胞生物,信号转导,细胞凋亡,表观遗传学, |
英文别名 | DNA (cytosine 5 ) methyltransferase 3 alpha; DNA (cytosine 5) methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3a; DNA methyltransferase HsaIIIA; DNA MTase HsaI; DNA MTase HsaIIIA; DNMT 3a; DNMT; DNMT3A2; M HsaIIIA; M.HsaIII |
反应物种(已验证) | Human,Rat |
反应物种(预测) | Mouse,Cow |
产品应用(已验证) | WB,IHC,FCM |
产品应用(可推荐) | IF,ELISA |
推荐稀释比例 | WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
克隆类型 | 多克隆 |
抗体来源 | Rabbit |
理论分子量 | 100 |
细胞定位 | 细胞核,细胞浆 |
性状 | Liquid |
免疫原 | KLH conjugated synthetic peptide derived from human Dnmt3a |
抗原表位 | 26-100/912 |
亚型 | IgG |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin. |
Tissue | Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. |
SIMILARITY | Belongs to the C5-methyltransferase family.
Contains 1 ADD domain. Contains 1 GATA-type zinc finger. Contains 1 PHD-type zinc finger. Contains 1 PWWP domain. |
SUBUNIT | Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with UBC9, PIAS1 and PIAS2. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD domain. Inter |
Function | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates |
Post-translational | Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. |
SWISS | Q9Y6K1 |
Gene ID | 1788 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016] |