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货号 | bs-0520R-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Rabbit Anti-PKA alpha + beta antibody |
中文名称 | Rabbit Anti-PKA alpha + beta antibody |
研究领域 | 细胞生物,信号转导,细胞凋亡,激酶和磷酸酶 |
英文别名 | PRKACA/PRKACB; PRKACA + PRKACB; PKA alpha + beta; PKA alpha; PKA beta; cAMP dependent protein kinase alpha catalytic subunit; cAMP dependent protein kinase beta catalytic subunit; cAMP dependent protein kinase catalytic subunit alpha; cAMP dependent prote |
反应物种(已验证) | Human,Mouse |
反应物种(预测) | Rat |
产品应用(已验证) | WB,ICC |
产品应用(可推荐) | IHC,IF,ELISA |
推荐稀释比例 | WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100, |
克隆类型 | 多克隆 |
抗体来源 | Rabbit |
理论分子量 | 40 |
细胞定位 | 细胞核,细胞浆,细胞膜 |
性状 | Liquid |
免疫原 | KLH conjugated synthetic peptide derived from human PRKACA/PRKACB |
抗原表位 | 17-100/351 |
亚型 | IgG |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Cytoplasm. Cell membrane. Nucleus. Mitochondrion. Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to m |
Tissue | Isoform 1 is ubiquitous. Isoform 2 is sperm specific. |
SIMILARITY | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. |
SUBUNIT | A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regul |
Function | Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subs |
Post-translational | Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.
Autophosphorylated. Phosphorylation is enhanced by vitamin K(2). Phosphorylated on threonine and serine residues. Phosphorylation on Thr-1 |
SWISS | P17612 |
Gene ID | 5566 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |