| Rabbit Anti-CXCR1 antibody |
| 反应物种（预测） |
Mouse,Dog,Cow,Rabbit |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,免疫学,细胞膜受体 |
| 标签 |
Array |
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Sample:
Lane 1: Human Jurkat cell Lysates
Lane 2: Human Raji cell Lysates
Primary: Anti-CXCR1 (bs-1009R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40kDa
Observed band size: 40kDa
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Sample:
HepG2(Human) Cell Lysate at 40 ug
Primary: Anti- CXCR1 (bs-1009R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD
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Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CXCR1/IL-8RA Polyclonal Antibody, Unconjugated(bs-1009R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID：AB_10857682
产品名称：Rabbit Anti-CXCR1 antibody
别名： C C; C C CKR 1; CCCKR1; CD 181; CD128; CD181; CDw128a; Chemokine (C X C motif) receptor 1; Chemokine (C X C) receptor 1; Chemokine receptor 1; CKR 1; CMKAR1; CXCR 1; High affinity interleukin 8 receptor A; IL 8 receptor type 1; IL 8R A; IL-8RA; IL8 recept
中文名称：细胞表面趋化因子受体1抗体
英文名称：Rabbit Anti-CXCR1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human CXCR1
抗原表位：186-280/350
抗原细胞定位：Cytoplasmic
SWISS：P25024
Gene ID ：3577
Human Gene ID：3577
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq, Jul 2008]
Function：Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system
Subcellular Location：Cell membrane.
Similarity：Belongs to the G-protein coupled receptor 1 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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