Rabbit Anti-CD133 antibody |
反应物种(预测) |
Rat,Fruit Fly |
产品应用(已验证) |
WB,FCM |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500, |
研究领域 |
肿瘤,细胞生物,免疫学,干细胞,细胞表面分子, |
标签 |
Array |
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Blank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature).
Primary Antibody (green line): Rabbit Anti-CD133 antibody (bs-0209R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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Sample:
Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 2: Lovo (Human) Cell Lysate at 30 ug
Lane 3: SW480 (Human) Cell Lysate at 30 ug
Primary: Anti-CD133 antigen (bs-0209R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110 kD
Observed band size: 120 kD
RRID:AB_11107865
产品名称:Rabbit Anti-CD133 antibody
别名: AC133; Antigen AC133; Hematopoietic stem cell antigen; hProminin; PROM1; Prominin I; Prominin like protein 1 precursor; Prominin mouse like 1; prominin1; PROML1; CD133 antigen; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4; PROM1_HUMAN.
中文名称:造血干细胞抗原CD133抗体
英文名称:Rabbit Anti-CD133 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human CD133
抗原表位:836-880/865
抗原细胞定位:Cytoplasmic
SWISS:O43490
Gene ID :8842
Human Gene ID:8842
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Function:Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regul
Subunit:Interacts with CDHR1 and with actin filaments.
Subcellular Location:Cell projection, cilium, photoreceptor outer segment. Isoform 1: Apical cell membrane; Multi-pass membrane protein. Cell projection, microvillus membrane; Multi-pass membrane protein. Note=Found in extracellular membrane particles in various body fluids s
Tissue Specificity:Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number
Post-translational modifications:Isoform 1 and isoform 2 are glycosylated.
DISEASE:Defects in PROM1 are the cause of retinitis pigmentosa type 41 (RP41) [MIM:612095]; also known as retinal degeneration autosomal recessive prominin-related. RP is a retinal dystrophy belonging to the group of pigmentary retinopathies. RP is characterized
Similarity:Belongs to the prominin family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.