Rabbit Anti-ICAM1 antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

订购号:bs-0608R

￥1180.00-3200.00

50ul

100ul

200ul

货期：现货

Rabbit Anti-ICAM1 antibody
反应物种（预测）	Rat
产品应用（已验证）	WB,IHC,FCM
产品应用（可尝试）	ICC,IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500,ICC=1:100-500,
研究领域	肿瘤,细胞生物,免疫学,信号转导,干细胞,细胞膜受体,细胞粘附分子,细胞表面分子,糖蛋白,细胞类型标志物,T-淋巴细胞,B-淋巴细胞,
标签	Array

Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/69 kD
Blank control: HUVEC cells(blue).
Primary Antibody:Rabbit Anti-CD54 antibody(bs-0608R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) .Primary antibody (bs-0608R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Blank control: mouse thymouses(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA(green).
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/69 kD
Blank control (blue line): A431 cells(blue).
Primary Antibody (green line): Rabbit Anti-ICAM1/PE-CY7 Conjugated antibody (bs-0608R-PE-CY7)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7 .
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃ . The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD54/ICAM-1 Polyclonal Antibody, Unconjugated(bs-0608R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/69 kD
Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-CD54 (bs-0608R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Hela KO ICAM1 (Human) Cell Lysate at 30 ug
Primary: Anti-ICAM1/CD54 (bs-0608R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: MCF-7 (Human) Cell Lysate at 30 ug
Primary: Anti-ICAM1/CD54 (bs-0608R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110/58 kD
Observed band size: 110/58 kD

产品信息

RRID：AB_10856895
产品名称：Rabbit Anti-ICAM1 antibody
别名： BB2; CD54 antigen; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2; MyD10; inter
中文名称：细胞间粘附分子-1（CD54）抗体
英文名称：Rabbit Anti-ICAM1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human CD54
抗原表位：201-300/537
抗原细胞定位：Extracellular
SWISS：P05362
Gene ID ：3383
Human Gene ID：3383

产品介绍

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
Function：ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In cas
Subunit：Homodimer (Probable). Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Binds to coxsackievirus A21 capsid proteins and acts as a receptor for t
Subcellular Location：Membrane; Single-pass type I membrane protein.
Post-translational modifications：Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Similarity：Belongs to the immunoglobulin superfamily. ICAM family.
ontains 5 Ig-like C2-type (immunoglobulin-like) domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.