| Rabbit Anti-Caspase-1 P10 antibody |
| 反应物种（预测） |
Rat |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,细胞凋亡 |
| 标签 |
Array |
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Sample: Mcf-7 Cell Lysate at 40 ug
Primary: Anti- Caspase-1 P10 (bs-0169R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 10/45 kD
Observed band size: 48 kD
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Tissue/cell: rat transplant lymphoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Caspase-1 Polyclonal Antibody, Unconjugated(bs-0169R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Caspase-1 Polyclonal Antibody, Unconjugated(bs-0169R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-1 P10) Polyclonal Antibody, Unconjugated (bs-0169R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-1 P10) Polyclonal Antibody, Unconjugated (bs-0169R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti-Caspase-1 P10 (bs-0169R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 10/45 kD
Observed band size: 45 kD
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Sample:
U-87MG(Human) Cell Lysate at 30 ug
Primary: Anti-Caspase-1 P10 (bs-0169R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 10/45 kD
Observed band size: 45 kD
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Sample:
Liver (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti- Caspase-1 P10 (bs-0169R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 10/45 kD
Observed band size: 35 kD
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Blank control:HL-60.
Primary Antibody (green line): Rabbit Anti-Caspase-1 P10 antibody (bs-0169R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_10858055
产品名称：Rabbit Anti-Caspase-1 P10 antibody
别名： caspase-1 isoform alpha p10 subunit; CASP 1; CASP1; Caspase 1 apoptosis related cysteine peptidase; Caspase 1 apoptosis related cysteine protease; Caspase1; ICE; IL 1 beta converting enzyme; IL 1BC; IL1B convertase; IL1BC; IL1BCE; Interleukin 1 beta conve
中文名称：天冬氨酸-胱氨酸特异性蛋白酶家族抗体
英文名称：Rabbit Anti-Caspase-1 P10 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Caspase-1
抗原表位：320-404/404
SWISS：P29466
Gene ID ：834
Human Gene ID：834
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq].
Function：Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding prote
Subunit：Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a compo
Subcellular Location：Cytoplasm.
Tissue Specificity：Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
Post-translational modifications：The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
Similarity：Belongs to the peptidase C14A family.
Contains 1 CARD domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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