| Rabbit Anti-14-3-3 Alpha + Beta + Gamma + Delta + Epsilon antibody |
| 反应物种（预测） |
Sheep,Fruit Fly,YEAST |
| 产品应用（已验证） |
WB,IHC,IF,FCM |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg /test.,IF=1:100-500, |
| 研究领域 |
细胞凋亡 |
| 标签 |
Array |
-
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (14-3-3 Alpha + Beta + Gamma + Delta + Epsilon) Polyclonal Antibody, Unconjugated (bs-1024R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
-
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (14-3-3 Alpha + Beta + Gamma + Delta + Epsilon) Polyclonal Antibody, Unconjugated (bs-1024R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
-
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-14-3-3 Alpha + Beta + Gamma + Delta + Epsilon antibody (bs-0237R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
-
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Uterus (Mouse) Lysate at 40 ug
Lane 3: Testis (Mouse) Lysate at 40 ug
Lane 4: Liver (Mouse) Lysate at 40 ug
Lane 5: Lung (Mouse) Lysate at 40 ug
Lane 6: Cerebrum (Rat) Lysate at 40 ug
Lane 7: Uterus (Rat) Lysate at 40 ug
Lane 8: Testis (Rat) Lysate at 40 ug
Lane 9: Liver (Rat) Lysate at 40 ug
Lane 10: Lung (Rat) Lysate at 40 ug
Lane 11: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 12: Siha (Human) Cell Lysate at 30 ug
Lane 13: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-14-3-3 Alpha+Beta+Gamma+Delta+Epsilon (bs-0237R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
-
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-14-3-3 Alpha + Beta + Gamma + Delta + Epsilon (bs-0237R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
-
Tissue/cell: human rectal carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-14-3-3 Polyclonal Antibody, Unconjugated(bs-0237R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
-
Sample:Brain Cell Lysate at 40 ug
Primary: Anti-14-3-3 Alpha + Beta + Gamma + Delta + Epsilon (bs-0237R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
-
Blank control (blue line): A549 (blue).
Primary Antibody (green line): Rabbit Anti-14-3-3 Alpha + Beta + Gamma + Delta + Epsilon antibody (bs-0237R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
-
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (14-3-3 Alpha + Beta + Gamma + Delta + Epsilon) Polyclonal Antibody, Unconjugated (bs-0237R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
RRID：AB_10859172
产品名称：Rabbit Anti-14-3-3 Alpha + Beta + Gamma + Delta + Epsilon antibody
别名： YWHAB; YWHAZ; 14-3-3 alpha/beta/Gamma/Delta/Epsilon; 14-3-3 alpha/beta; 14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein zeta; KCIP 1; Protein 1054; Protein kinase C inhibitor protein 1; 14 3 3 protein delta; 14 3 3 protein gamma; 14 3 3 pro
中文名称：14-3-3蛋白/14-3-3 α/β/γ/δ/ε亚型抗体
英文名称：Rabbit Anti-14-3-3 Alpha + Beta + Gamma + Delta + Epsilon antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human 14-3-3
抗原表位：174-245/245
SWISS：P31946
Gene ID ：7529
Human Gene ID：7529
14-3-3 are activates tyrosine and tryptophan hydroxylases in the presence of Ca (2+)/calmodulin-dependent protein kinase II, and strongly activates protein kinase C. Is probably a multifunctional regulator of the cell signaling processes mediated by both kinases. Activates the ADP-ribosyltransferase (exoS) activity of bacterial origin. 14-3-3 proteins are localized in neurons, and are axonally transported to the nerve terminals. They may be also present, at lower levels, in various other eukaryotic tissues. It belongs to the 14-3-3 family.
This antibody is reactive with 14-3-3 Alpha, Beta, Gamma, Delta, Epsilon.
Function：Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the
Subunit：Interacts with CDK16 and BSPRY. Interacts with WEE1 (C-terminal). Interacts with SAMSN1. Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with Thr-phosphorylated ITGB2. Interacts with BCL2L11. Homodimer. He
Subcellular Location：Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes.
Post-translational modifications：The delta, brain-specific form differs from the zeta form in being phosphorylated. Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and
Similarity：Belongs to the 14-3-3 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
说明书
联系我们
打印此页面
收藏