| Rabbit Anti-Osteocalcin antibody |
| 反应物种（预测） |
Human,Rat,Dog,Pig,Cow,Horse |
| 产品应用（已验证） |
IHC |
| 产品应用（可尝试） |
WB,ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
细胞生物,信号转导,干细胞,细胞外基质, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (bs-4917R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
-
citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (bs-4917R) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin (bs-0437P-Cy3) at[1:500] for 40 minutes and DAPI staining of the nu Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium clei.
RRID：RRID
产品名称：Rabbit Anti-Osteocalcin antibody
别名： Osteocalcin Propeptide; Gamma-carboxyglutamic acid-containing protein; Bone Gla-protein; BGLAP; Bone gamma carboxyglutamate protein; BGLAP; BGP; Bone gamma carboxyglutamate gla protein osteocalcin; Bone gamma carboxyglutamate protein; Bone Gla protein; Ga
中文名称：骨钙蛋白/骨钙素抗体
英文名称：Rabbit Anti-Osteocalcin antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Osteocalcin
抗原表位：21-100/100
SWISS：P02818
Gene ID ：632
Human Gene ID：632
Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite.
Function：Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Subcellular Location：Secreted.
Post-translational modifications：Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
Similarity：Belongs to the osteocalcin/matrix Gla protein family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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