货号 |
bs-8394R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-Fbxw7 antibody |
中文名称 |
Rabbit Anti-Fbxw7 antibody |
研究领域 |
肿瘤,细胞生物,免疫学,神经生物学 |
英文别名 |
AGO; Archipelago homolog; Archipelago, Drosophila, homolog of antibody CDC4; DKFZp686F23254; F box and WD 40 domain protein 7 (archipelago homolog, Drosophila); F box and WD 40 domain protein 7; F box and WD repeat domain containing 7; F box protein FBW7; |
反应物种(已验证) |
Human,Mouse,Rat |
反应物种(预测) |
Dog,Pig,Cow,Horse |
产品应用(已验证) |
IHC,ICC,FCM |
产品应用(可推荐) |
IF,ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:50-200,ICC=1:100, |
克隆类型 |
多克隆 |
抗体来源 |
Rabbit |
理论分子量 |
78 |
细胞定位 |
细胞核,细胞浆 |
性状 |
Liquid |
免疫原 |
KLH conjugated synthetic peptide derived from human Fbxw7/CDC4 |
抗原表位 |
501-600/707 |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
Isoform 1: Nucleus, nucleoplasm. Isoform 2: Cytoplasm. Isoform 4: Nucleus, nucleolus. Nucleus. |
Tissue |
Isoform 1 is widely expressed. Isoform 4 is expressed in brain. |
SIMILARITY |
Contains 1 F-box domain.
Contains 7 WD repeats. |
SUBUNIT |
Component of the SCF(FBXW7) complex consisting of CUL1, RBX1, SKP1 and FBXW7. Interacts with PSEN1, cyclin E, NOTCH1 NICD, NOTCH4 NICD and SKP1. Interacts with MYC (when phosphorylated). Isoform 1 interacts with USP28, leading to counteract ubiquitination |
Function |
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target prot |
Post-translational |
Phosphorylated upon DNA damage, probably by ATM or ATR. |
SWISS |
Q969H0 |
Gene ID |
55294 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
The F-box protein family is characterized by an approximately 40 amino acid motif known as the F-box. F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. One family member, Cdc4, also known as AGO, FBW7, FBXW7, FBX30, SEL10, and FLJ11071, maps to human chromosome 4q31.3. Alternative splicing of this gene generates four transcript variants. In addition to an F-box, Cdc4 contains seven tandem WD40 repeats. Cdc4 binds directly to cyclin E and targets cyclin E for ubiquitin-mediated degradation. Mutations of the Cdc4 gene are detected in ovarian and breast cancer cell lines, suggesting that the gene may be involved in the pathogenesis of human cancers. |