| Rabbit Anti-Fbxw7 antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Horse |
| 产品应用（已验证） |
IHC,ICC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:50-200,ICC=1:100, |
| 研究领域 |
肿瘤,细胞生物,免疫学,神经生物学 |
| 标签 |
Array |
-
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Fbxw7/CDC4 Polyclonal Antibody, Unconjugated(bs-8394R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
-
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fbxw7) Polyclonal Antibody, Unconjugated (bs-8394R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
-
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fbxw7) Polyclonal Antibody, Unconjugated (bs-8394R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
-
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Fbxw7) polyclonal Antibody, Unconjugated (bs-8394R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
-
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-Fbxw7 antibody (bs-8394R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：RRID
产品名称：Rabbit Anti-Fbxw7 antibody
别名： AGO; Archipelago homolog; Archipelago, Drosophila, homolog of antibody CDC4; DKFZp686F23254; F box and WD 40 domain protein 7 (archipelago homolog, Drosophila); F box and WD 40 domain protein 7; F box and WD repeat domain containing 7; F box protein FBW7;
中文名称：Fbxw7蛋白抗体
英文名称：Rabbit Anti-Fbxw7 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Fbxw7/CDC4
抗原表位：501-600/707
SWISS：Q969H0
Gene ID ：55294
Human Gene ID：55294
The F-box protein family is characterized by an approximately 40 amino acid motif known as the F-box. F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. One family member, Cdc4, also known as AGO, FBW7, FBXW7, FBX30, SEL10, and FLJ11071, maps to human chromosome 4q31.3. Alternative splicing of this gene generates four transcript variants. In addition to an F-box, Cdc4 contains seven tandem WD40 repeats. Cdc4 binds directly to cyclin E and targets cyclin E for ubiquitin-mediated degradation. Mutations of the Cdc4 gene are detected in ovarian and breast cancer cell lines, suggesting that the gene may be involved in the pathogenesis of human cancers.
Function：Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target prot
Subunit：Component of the SCF(FBXW7) complex consisting of CUL1, RBX1, SKP1 and FBXW7. Interacts with PSEN1, cyclin E, NOTCH1 NICD, NOTCH4 NICD and SKP1. Interacts with MYC (when phosphorylated). Isoform 1 interacts with USP28, leading to counteract ubiquitination
Subcellular Location：Isoform 1: Nucleus, nucleoplasm. Isoform 2: Cytoplasm. Isoform 4: Nucleus, nucleolus. Nucleus.
Tissue Specificity：Isoform 1 is widely expressed. Isoform 4 is expressed in brain.
Post-translational modifications：Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity：Contains 1 F-box domain.
Contains 7 WD repeats.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
说明书
联系我们
打印此页面
收藏