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货号 | bs-3456R-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Rabbit Anti-Phospho-TrkA (Tyr496) +TrkB(Tyr516) antibody |
中文名称 | Rabbit Anti-Phospho-TrkA (Tyr496) +TrkB(Tyr516) antibody |
产品标签 | 磷酸化抗体 |
研究领域 | 细胞生物,免疫学,神经生物学,信号转导,转录调节因子,激酶和磷酸酶 |
英文别名 | TrkA (Phospho-Tyr496); TrkA (Phospho Tyr496); TrkA (Phospho-Y496); TrkA (phospho Y496); p-TrkA(Tyr496); TrkB (Phospho-Tyr516); TrkB (Phospho Tyr516); TrkB (Phospho-Y516); p-TrkB(Tyr516); CIPA; CIPA; DKFZp781I14186; gp140trk; High affinity nerve growth fac |
反应物种(已验证) | Human,Mouse |
反应物种(预测) | Rat |
产品应用(已验证) | WB,FCM |
产品应用(可推荐) | IHC,IF,ELISA |
推荐稀释比例 | WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
克隆类型 | 多克隆 |
抗体来源 | Rabbit |
理论分子量 | 90 |
细胞定位 | 细胞浆,细胞膜 |
性状 | Liquid |
免疫原 | KLH conjugated Synthesised phosphopeptide derived from human TrkA around the phosphorylation site of Tyr496 |
抗原表位 | PQ(p-Y)F |
亚型 | IgG |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Cell membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Note=Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. |
Tissue | Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors. |
SIMILARITY | Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 2 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 protein |
SUBUNIT | Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Homodimerization is induced by binding of a NGF dimer. Interacts with SQSTM1; bridges NTRK1 to NGFR. Forms a ternary complex with NGFR and KIDINS220; |
Function | Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF whic |
Post-translational | Ligand-mediated autophosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1.
N-glycosylated (Probable). Isoform TrkA-I is N-glycosylated. Ubiquitinated. |
DISEASE | Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis (CIPA) [MIM:256800]. CIPA is characterized by a congenital insensitivity to pain, anhidrosis (absence of sweating), absence of reaction to noxious stimuli, self-mutilating be |
SWISS | P04629 |
Gene ID | 4914 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | The Trk family of nerve growth factor receptors includes Trk A(also referfed to as Trk A gp140),Trk B and Trk C. The prototype member of this gene family, Trk A, encodes a 140 kDa cell surface receptor , gp140, the expression of which is restricted in vivo to neurons of the sensory spinal and cranial gangliaof neurocrest origin. Nerve growth factor (NGF) stimulates tyrosine phosphorylation of Trk gp 140 in neural cell lines and in embryonic dorsal root ganglia. By comparison, BDNF and to a lesser extent, NT-3, but not NGF, can induce tyrosine phophorylayion of Trk B gp 145. The third member of the Trk receptor family, Trk C incodes a 140 kDa protein, Trk C gp140, that is preferentially expressed in brain tissue and primarily functions as a receptor for NT-3.An additional component of the Trk receptor complex, NGFR p175, binds to neurotrophic factors with low affinity but is required for efficient signaling. NGFR p175 accelerates Trk activation and may recruit downstream dffector molecules to the ligand-bound receptor complex. |