Rabbit Anti-Phospho-TrkA (Tyr496) +TrkB(Tyr516) antibody |
反应物种(预测) |
Rat |
产品应用(已验证) |
WB,FCM |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
研究领域 |
细胞生物,免疫学,神经生物学,信号转导,转录调节因子,激酶和磷酸酶 |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TrkA(Tyr496) +TrkB(Tyr516)) Polyclonal Antibody, Unconjugated (bs-3456R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Sample:
Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 2: K562 (Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-TrkA (Tyr496) +TrkB(Tyr516) (bs-3456R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
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Blank control: K562.
Primary Antibody (green line): Rabbit Anti-Phospho-TrkA (Tyr496)+TrkB(Tyr516)antibody (bs-3456R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID:AB_10854906
产品名称:Rabbit Anti-Phospho-TrkA (Tyr496) +TrkB(Tyr516) antibody
别名: TrkA (Phospho-Tyr496); TrkA (Phospho Tyr496); TrkA (Phospho-Y496); TrkA (phospho Y496); p-TrkA(Tyr496); TrkB (Phospho-Tyr516); TrkB (Phospho Tyr516); TrkB (Phospho-Y516); p-TrkB(Tyr516); CIPA; CIPA; DKFZp781I14186; gp140trk; High affinity nerve growth fac
中文名称:磷酸化酪氨酸激酶A抗体
英文名称:Rabbit Anti-Phospho-TrkA (Tyr496) +TrkB(Tyr516) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human TrkA around the phosphorylation site of Tyr496
抗原表位:PQ(p-Y)F
SWISS:P04629
Gene ID :4914
Human Gene ID:4914
The Trk family of nerve growth factor receptors includes Trk A(also referfed to as Trk A gp140),Trk B and Trk C. The prototype member of this gene family, Trk A, encodes a 140 kDa cell surface receptor , gp140, the expression of which is restricted in vivo to neurons of the sensory spinal and cranial gangliaof neurocrest origin. Nerve growth factor (NGF) stimulates tyrosine phosphorylation of Trk gp 140 in neural cell lines and in embryonic dorsal root ganglia. By comparison, BDNF and to a lesser extent, NT-3, but not NGF, can induce tyrosine phophorylayion of Trk B gp 145. The third member of the Trk receptor family, Trk C incodes a 140 kDa protein, Trk C gp140, that is preferentially expressed in brain tissue and primarily functions as a receptor for NT-3.An additional component of the Trk receptor complex, NGFR p175, binds to neurotrophic factors with low affinity but is required for efficient signaling. NGFR p175 accelerates Trk activation and may recruit downstream dffector molecules to the ligand-bound receptor complex.
Function:Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF whic
Subunit:Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Homodimerization is induced by binding of a NGF dimer. Interacts with SQSTM1; bridges NTRK1 to NGFR. Forms a ternary complex with NGFR and KIDINS220;
Subcellular Location:Cell membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Note=Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport.
Tissue Specificity:Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
Post-translational modifications:Ligand-mediated autophosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1.
N-glycosylated (Probable). Isoform TrkA-I is N-glycosylated.
Ubiquitinated.
DISEASE:Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis (CIPA) [MIM:256800]. CIPA is characterized by a congenital insensitivity to pain, anhidrosis (absence of sweating), absence of reaction to noxious stimuli, self-mutilating be
Similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 protein
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.