货号 |
bs-5396R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-phospho-IRS1 (Ser312) antibody |
中文名称 |
Rabbit Anti-phospho-IRS1 (Ser312) antibody |
产品标签 |
磷酸化抗体 |
研究领域 |
肿瘤,免疫学,信号转导,转录调节因子,细胞膜受体,糖尿病,细胞膜蛋白, |
英文别名 |
IRS1 (phospho S312); IRS-1 (Phospho-Ser312); p-IRS1; IRS1(phospho-Ser789); IRS1_HUMAN; Insulin receptor substrate 1; IRS-1; IRS 1; |
反应物种(已验证) |
Human,Rat |
反应物种(预测) |
Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep |
产品应用(已验证) |
IHC,FCM |
产品应用(可推荐) |
ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,Flow Cyt=0.2μg/Test, |
克隆类型 |
多克隆 |
抗体来源 |
Rabbit |
理论分子量 |
132 |
细胞定位 |
细胞膜 |
性状 |
Liquid |
免疫原 |
KLH conjugated Synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser312 |
抗原表位 |
AT(p-S)PA |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
Membrane; Single-pass type I membrane protein. |
Tissue |
Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vasc |
SIMILARITY |
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. |
SUBUNIT |
Interacts with UBTF and PIK3CA. Interacts (via phosphorylated YXXM motifs) with PIK3R1. Interacts with ROCK1 and FER. Interacts (via PH domain) with PHIP. Interacts with GRB2. Interacts with SOCS7. Interacts (via IRS-type PTB domain) with IGF1R and INSR ( |
Function |
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosp |
Post-translational |
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding. Phosphoryl |
DISEASE |
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. |
SWISS |
P35568 |
Gene ID |
3667 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS1 degradation pathways are not well understood. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS1 phosphorylation and subsequent degradation an attractive therapeutic target. To date there have been four subtypes identified: IRS1, 2, 3 and 4, with IRS1 being widely expressed. |