| Rabbit Anti-phospho-IRS1 (Ser312) antibody |
| 反应物种（预测） |
Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep |
| 产品应用（已验证） |
IHC,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,Flow Cyt=0.2μg/Test, |
| 研究领域 |
肿瘤,免疫学,信号转导,转录调节因子,细胞膜受体,糖尿病,细胞膜蛋白, |
| 标签 |
Array |
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Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti- phospho-IRS1 (Ser312) antibody (bs-5396R), Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-IRS1 (Ser312)) Polyclonal Antibody, Unconjugated (bs-5396R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-phospho-IRS1 (Ser323) antibody (bs-3152R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_11066715
产品名称：Rabbit Anti-phospho-IRS1 (Ser312) antibody
别名： IRS1 (phospho S312); IRS-1 (Phospho-Ser312); p-IRS1; IRS1(phospho-Ser789); IRS1_HUMAN; Insulin receptor substrate 1; IRS-1; IRS 1;
中文名称：磷酸化胰岛素受体底物-1抗体
英文名称：Rabbit Anti-phospho-IRS1 (Ser312) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser312
抗原表位：AT(p-S)PA
SWISS：P35568
Gene ID ：3667
Human Gene ID：3667
Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS1 degradation pathways are not well understood. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS1 phosphorylation and subsequent degradation an attractive therapeutic target. To date there have been four subtypes identified: IRS1, 2, 3 and 4, with IRS1 being widely expressed.
Function：May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosp
Subunit：Interacts with UBTF and PIK3CA. Interacts (via phosphorylated YXXM motifs) with PIK3R1. Interacts with ROCK1 and FER. Interacts (via PH domain) with PHIP. Interacts with GRB2. Interacts with SOCS7. Interacts (via IRS-type PTB domain) with IGF1R and INSR (
Subcellular Location：Membrane; Single-pass type I membrane protein.
Tissue Specificity：Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vasc
Post-translational modifications：Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding. Phosphoryl
DISEASE：Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
Similarity：Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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