货号 |
bs-2967R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-MBP tag antibody |
中文名称 |
Rabbit Anti-MBP tag antibody |
产品标签 |
标签抗体 |
研究领域 |
细胞生物,免疫学,糖蛋白, |
英文别名 |
MBP-tag; MBP; MMBP; Maltose Binding Protein; Maltose binding protein tag; ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; Periplasmic maltose binding prote |
反应物种(预测) |
Escherichia Coli,fusion protein |
产品应用(已验证) |
WB |
产品应用(可推荐) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:5000-20000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
克隆类型 |
多克隆 |
抗体来源 |
Rabbit |
理论分子量 |
44 |
细胞定位 |
细胞浆 |
性状 |
Liquid |
免疫原 |
KLH conjugated synthetic peptide derived from E. coli Maltose Binding Protein |
抗原表位 |
1-100/396 |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
Periplasm. |
SIMILARITY |
Belongs to the bacterial solute-binding protein 1 family. |
Function |
Maltose binding protein-MBP is E. coli malE gene encoding the protein, bacterial member of the maltose transport system. malE gene product can integrate with a variety of proteins, it is an effective medium for expression studies. subunit antibody. |
SWISS |
P0AEX9 |
Gene ID |
1039564 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein. |