Rabbit Anti-MBP tag antibody |
反应物种(预测) |
Escherichia Coli,fusion protein |
产品应用(已验证) |
WB |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:5000-20000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
细胞生物,免疫学,糖蛋白, |
标签 |
Array |
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Sample:
Lane 1: MBP-tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-33131P) at 4 ug
Primary:
Anti-MBP tag (bs-2967R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD
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Sample:
Lane 1: MBP tagged cytochrome C (Full Length) Protein (bs-41232P) at 4 ug
Lane 2: MBP tagged caspase 3 p17 (143-213) Protein (bs-41233P) at 4 ug
Primary: Anti-MBP tag (bs-2967R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60-70 kD
Observed band size: 62 kD
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Sample:
Lane 1: MBP-Tagged Fusion Protein (bs- 41233P) at 300ng
Primary: Anti-MBP tag (bs-2967R) at 1/20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 60 kDa
RRID:RRID
产品名称:Rabbit Anti-MBP tag antibody
别名: MBP-tag; MBP; MMBP; Maltose Binding Protein; Maltose binding protein tag; ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; Periplasmic maltose binding prote
中文名称:麦芽糖结合蛋白抗体
英文名称:Rabbit Anti-MBP tag antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from E. coli Maltose Binding Protein
抗原表位:1-100/396
SWISS:P0AEX9
Gene ID :1039564
Human Gene ID:1039564
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.
Function:Maltose binding protein-MBP is E. coli malE gene encoding the protein, bacterial member of the maltose transport system. malE gene product can integrate with a variety of proteins, it is an effective medium for expression studies. subunit antibody.
Subcellular Location:Periplasm.
Similarity:Belongs to the bacterial solute-binding protein 1 family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.