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货号 | bs-18747R-1 |
品牌 | |
浓度 | |
货期 | 现货 |
英文名称 | Rabbit Anti-phospho-MDM2 (Ser188 + Ser186) antibody |
中文名称 | Rabbit Anti-phospho-MDM2 (Ser188 + Ser186) antibody |
产品标签 | 磷酸化抗体 |
研究领域 | 细胞生物,细胞周期蛋白,表观遗传学,泛素, |
英文别名 | MDM2 (phospho S188 + S186); p-MDM2 (phospho S188 + S186); ACTFS; Double minute 2 protein; E3 ubiquitin-protein ligase Mdm2; Hdm 2; Hdm2; HDMX; MDM 2; MDM2; MDM2 oncogene E3 ubiquitin protein ligase; Mdm2 p53 E3 ubiquitin protein ligase homolog; |
反应物种(已验证) | Human |
产品应用(已验证) | FCM |
产品应用(可推荐) | WB,IHC,ICC,IF,ELISA |
推荐稀释比例 | WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500,ICC=1:100, |
克隆类型 | 多克隆 |
抗体来源 | Rabbit |
理论分子量 | 55 |
细胞定位 | 细胞核,细胞浆 |
性状 | Liquid |
免疫原 | KLH conjugated synthesised phosphopeptide derived from human MDM2 around the phosphorylation site of Ser188 + Ser186 |
抗原表位 | HK(p-S)D(p-S)IS |
亚型 | IgG |
纯化方法 | affinity purified by Protein A |
SUBCELLULAR | Nucleus > nucleoplasm. Cytoplasm. Nucleus > nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be |
Tissue | Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues. |
SIMILARITY | Belongs to the MDM2/MDM4 family. Contains 1 RanBP2-type zinc finger. Contains 1 RING-type zinc finger. Contains 1 SWIB domain. |
Function | E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an |
Post-translational | Phosphorylated in response to ionizing radiation in an ATM-dependent manner. Auto-ubiquitinated; which leads to proteasomal degradation. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitinilation and degradation of p53/TP53. Deubiq |
DISEASE | Note=Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Fou |
SWISS | Q00987 |
Gene ID | 4193 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 | This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013] |