| Rabbit Anti-phospho-MDM2 (Ser188 + Ser186) antibody |
| 产品应用（已验证） |
FCM |
| 产品应用（可尝试） |
WB,IHC,ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500,ICC=1:100, |
| 研究领域 |
细胞生物,细胞周期蛋白,表观遗传学,泛素, |
| 标签 |
Array |
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Blank control:Molt4.
Primary Antibody (green line): Rabbit Anti-phospho-MDM2 (Ser188 + Ser186) antibody (bs-18747R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：RRID
产品名称：Rabbit Anti-phospho-MDM2 (Ser188 + Ser186) antibody
别名： MDM2 (phospho S188 + S186); p-MDM2 (phospho S188 + S186); ACTFS;
Double minute 2 protein;
E3 ubiquitin-protein ligase Mdm2;
Hdm 2;
Hdm2;
HDMX;
MDM 2;
MDM2;
MDM2 oncogene E3 ubiquitin protein ligase;
Mdm2 p53 E3 ubiquitin protein ligase homolog;
中文名称：磷酸化双微体2癌基因抗体
英文名称：Rabbit Anti-phospho-MDM2 (Ser188 + Ser186) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised phosphopeptide derived from human MDM2 around the phosphorylation site of Ser188 + Ser186
抗原表位：HK(p-S)D(p-S)IS
SWISS：Q00987
Gene ID ：4193
Human Gene ID：4193
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]
Function：E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an
Subcellular Location：Nucleus > nucleoplasm. Cytoplasm. Nucleus > nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be
Tissue Specificity：Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
Post-translational modifications：Phosphorylated in response to ionizing radiation in an ATM-dependent manner.
Auto-ubiquitinated; which leads to proteasomal degradation. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitinilation and degradation of p53/TP53. Deubiq
DISEASE：Note=Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Fou
Similarity：Belongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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