货号 |
bsm-33133M-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Mouse Anti-TAP-Tag antibody |
中文名称 |
Mouse Anti-TAP-Tag antibody |
产品标签 |
标签抗体 |
英文别名 |
TAP Tag; TAPTag; Tandem affinity purification |
反应物种(预测) |
Species independent |
产品应用(已验证) |
WB |
产品应用(可推荐) |
ELISA |
推荐稀释比例 |
WB=1:1000-5000,Elisa=1:5000-10000, |
克隆类型 |
单克隆 |
克隆号 |
Mix-mA™ |
抗体来源 |
Mouse |
性状 |
Liquid |
免疫原 |
Recombinant TAP-Tag |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein G |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
Tandem affinity purification (TAP) is a purification technique for studying protein–protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix. |