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Mouse Anti-TAP-Tag antibody
单克隆  |   CloneNo.: Mix-mA™  |   SKU:bsm-33133M

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货号:bs-3351R
¥1280
订购号:bsm-33133M
¥-4000.00
货期:现货
Mouse Anti-TAP-Tag antibody
反应物种(预测)

Species independent

产品应用(已验证)

WB

产品应用(可尝试)

ELISA

推荐稀释比例

WB=1:1000-5000,Elisa=1:5000-10000,

标签

Array

  • Sample:
    Lane 1: TAP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug
    Lane 2: TAP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug
    Primary: Anti-TAP-Tag (bsm-33133M) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 51 kD
    Observed band size: 51 kD

产品信息

RRID:RRID
产品名称:Mouse Anti-TAP-Tag antibody
别名: TAP Tag; TAPTag; Tandem affinity purification
中文名称:TAP-Tag(标签)单克隆抗体
英文名称:Mouse Anti-TAP-Tag antibody
抗体来源: Mouse
克隆类型:单克隆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein G
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免 疫 原:Recombinant TAP-Tag
Human Gene ID:TAP Tag

产品介绍

Tandem affinity purification (TAP) is a purification technique for studying protein–protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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