货号 |
bsm-52205R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-Phospho-Smad3 (Ser423 + Ser425) antibody |
中文名称 |
Rabbit Anti-Phospho-Smad3 (Ser423 + Ser425) antibody |
产品标签 |
磷酸化抗体,重组兔单抗 |
研究领域 |
肿瘤,细胞生物,免疫学,信号转导,干细胞,细胞凋亡,转录调节因子,表观遗传学, |
英文别名 |
Smad3 (phospho Ser423/425); Smad3 (phospho S423 + S425); p-Smad3 (Ser423 + Ser425); hMAD 3; hSMAD3; HSPC193; JV15 2; JV152; MAD (mothers against decapentaplegic Drosophila) homolog 3; MAD3; MADH 3; MADH3; Mothers against decapentaplegic homolog 3; Mothers |
反应物种(已验证) |
Human,Mouse |
反应物种(预测) |
Rat,Pig |
产品应用(已验证) |
WB |
产品应用(可推荐) |
IHC,IF |
推荐稀释比例 |
WB=1:500-1000,IHC-P=1:20-200,IHC-F=1:20-200,IF=1:20-200, |
克隆类型 |
单克隆 |
克隆号 |
5C5 |
抗体来源 |
Rabbit |
理论分子量 |
47 |
细胞定位 |
细胞核,细胞浆 |
性状 |
Liquid |
免疫原 |
KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 |
抗原表位 |
CS(p-S)V(p-S) |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the |
SIMILARITY |
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. |
SUBUNIT |
Monomer; in the absence of TGF-beta. Homooligomer; in the presence of TGF-beta. Heterotrimer; forms a heterotrimer in the presence of TGF-beta consisting of two molecules of C-terminally phosphorylated SMAD2 or SMAD3 and one of SMAD4 to form the transcrip |
Function |
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes th |
Post-translational |
Phosphorylated on serine and threonine residues. Enhanced phosphorylation in the linker region on Thr-179, Ser-204 and Ser-208 on EGF AND TGF-beta treatment. Ser-208 is the main site of MAPK-mediated phosphorylation. CDK-mediated phosphorylation occurs in |
DISEASE |
Defects in SMAD3 may be a cause of colorectal cancer (CRC) [MIM:114500]. Defects in SMAD3 are the cause of Loeys-Dietz syndrome type 1C (LDS1C) [MIM:613795]. An aortic aneurysm syndrome with widespread systemic involvement. The disorder is characterized b |
SWISS |
P84022 |
Gene ID |
4088 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling. |