Rabbit Anti-Phospho-Smad3 (Ser423 + Ser425) antibody |
反应物种(预测) |
Rat,Pig |
产品应用(已验证) |
WB |
产品应用(可尝试) |
IHC,IF |
推荐稀释比例 |
WB=1:500-1000,IHC-P=1:20-200,IHC-F=1:20-200,IF=1:20-200, |
研究领域 |
肿瘤,细胞生物,免疫学,信号转导,干细胞,细胞凋亡,转录调节因子,表观遗传学, |
标签 |
Array |
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Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- Phospho-Smad3 (Ser423 + Ser425) (bsm-52205R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 50 kD
-
Sample:
Lung (Mouse) Lysate at 40 ug
A549(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- Phospho-Smad3 (Ser423 + Ser425) (bsm-52205R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 55 kD
-
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-Smad3 (Ser423 + Ser425) (bsm-52205R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
-
Sample:
SiHa(Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-Smad3 (Ser423 + Ser425) (bsm-52205R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
RRID:RRID
产品名称:Rabbit Anti-Phospho-Smad3 (Ser423 + Ser425) antibody
别名: Smad3 (phospho Ser423/425); Smad3 (phospho S423 + S425); p-Smad3 (Ser423 + Ser425); hMAD 3; hSMAD3; HSPC193; JV15 2; JV152; MAD (mothers against decapentaplegic Drosophila) homolog 3; MAD3; MADH 3; MADH3; Mothers against decapentaplegic homolog 3; Mothers
中文名称:磷酸化细胞信号转导分子SMAD3重组兔单克隆抗体
英文名称:Rabbit Anti-Phospho-Smad3 (Ser423 + Ser425) antibody
抗体来源: Rabbit
克隆类型:单克隆
细胞定位:细胞核,细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425
抗原表位:CS(p-S)V(p-S)
SWISS:P84022
Gene ID :4088
Human Gene ID:4088
Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.
Function:Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes th
Subunit:Monomer; in the absence of TGF-beta. Homooligomer; in the presence of TGF-beta. Heterotrimer; forms a heterotrimer in the presence of TGF-beta consisting of two molecules of C-terminally phosphorylated SMAD2 or SMAD3 and one of SMAD4 to form the transcrip
Subcellular Location:Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the
Post-translational modifications:Phosphorylated on serine and threonine residues. Enhanced phosphorylation in the linker region on Thr-179, Ser-204 and Ser-208 on EGF AND TGF-beta treatment. Ser-208 is the main site of MAPK-mediated phosphorylation. CDK-mediated phosphorylation occurs in
DISEASE:Defects in SMAD3 may be a cause of colorectal cancer (CRC) [MIM:114500]. Defects in SMAD3 are the cause of Loeys-Dietz syndrome type 1C (LDS1C) [MIM:613795]. An aortic aneurysm syndrome with widespread systemic involvement. The disorder is characterized b
Similarity:Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.