货号 |
bsm-51587M-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Mouse Anti-SP1 antibody |
中文名称 |
Mouse Anti-SP1 antibody |
研究领域 |
染色质和核信号,信号转导,干细胞,转录调节因子,新陈代谢,锌指蛋白,表观遗传学, |
英文别名 |
Sp1 transcription factor isoform a; TSFP1; TSFP 1; Specificity protein 1; Transcription factor Sp1; SP1_HUMAN. |
反应物种(已验证) |
Human |
产品应用(已验证) |
WB,IHC,ICC,FCM |
产品应用(可推荐) |
IF |
推荐稀释比例 |
WB=1:500-1000,IHC-P=1:50-100,Flow Cyt=1:25,IF=1:100-500,ICC=1:25, |
克隆类型 |
单克隆 |
克隆号 |
Y5R2 |
抗体来源 |
Mouse |
理论分子量 |
81 |
细胞定位 |
细胞核,细胞浆 |
性状 |
Liquid |
免疫原 |
Recombinant human SP1 |
亚型 |
IgG1,κ |
纯化方法 |
affinity purified by Protein G |
SUBCELLULAR |
Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location. |
Tissue |
Up-regulated in adenocarcinomas of the stomach (at protein level). |
SIMILARITY |
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. |
SUBUNIT |
Interacts with ATF7IP, ATF7IP2, BAHD1, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein. Interacts with HIV-1 Vpr; the interaction is inhibited by SP1 O-glycosylation. Interacts with SV40 VP2/3 proteins. Interacts with SV40 m |
Function |
Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes su |
Post-translational |
Phosphorylated on multiple serine and threonine residues. Phosphorylation is coupled to ubiquitination, sumoylation and proteolytic processing. Phosphorylation on Ser-59 enhances proteolytic cleavage. Phosphorylation on Ser-7 enhances ubiquitination and p |
SWISS |
P08047 |
Gene ID |
6667 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
Profound changes in patterns of gene expression can result from relatively small changes in the concentrations of sequence specific transcription factors. Synergistic interaction between factors bound to different sites within a transcriptional control region is supported by the work of Courey et al. (1989). Sp1 is a sequence specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. SP1 can interact with G/C rich motifs from serotonin receptor promoter. Kadonaga et al. (1987) cloned the human Sp1 cDNA and showed that it has contiguous zinc finger motifs and requires zinc for sequence specific binding to DNA. Altername:Sp1 transcription factor isoform a; TSFP1; TSFP 1; Specificity protein 1; Transcription factor Sp1. |