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Mouse Anti-SP1 antibody
单克隆  |   CloneNo.: Y5R2  |   SKU:bsm-51587M

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货号:bs-3351R
¥1280
订购号:bsm-51587M
¥1150.00-1880.00
货期:现货
Mouse Anti-SP1 antibody
产品应用(已验证)

WB,IHC,ICC,FCM

产品应用(可尝试)

IF

推荐稀释比例

WB=1:500-1000,IHC-P=1:50-100,Flow Cyt=1:25,IF=1:100-500,ICC=1:25,

研究领域

染色质和核信号,信号转导,干细胞,转录调节因子,新陈代谢,锌指蛋白,表观遗传学,

标签

Array

  • Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (SP1) monoclonal Antibody, Unconjugated (bsm-51587M) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes,Alexa Fluor® 555 conjugated with Phalloidin(red) was used to stain the cell Cytoplasmic actin.
  • Blank control: Hela.
    Primary Antibody (green line): Mouse Anti-SP1 antibody (bsm-51587M)
    Dilution: 1:25;
    Isotype Control Antibody (blue line): Mouse IgG Secondary Antibody : Goat anti-mouse IgG-AF488
    Dilution: 1:400 Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
  • Sample:
    Lane 1: Hela cell lysates
    Lane 2: Ramos cell lysates
    Primary: Anti-SP1 (bsm-51587M) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 81 kD
    Observed band size: 105 kD
  • Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SP1 ) Monoclonal Antibody, Unconjugated (bsm-51587M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

产品信息

RRID:RRID
产品名称:Mouse Anti-SP1 antibody
别名: Sp1 transcription factor isoform a; TSFP1; TSFP 1; Specificity protein 1; Transcription factor Sp1; SP1_HUMAN.
中文名称:转录生长因子SP1单克隆抗体
英文名称:Mouse Anti-SP1 antibody
抗体来源: Mouse
克隆类型:单克隆
细胞定位:细胞核,细胞浆
性 状:Liquid
亚 型:IgG1,κ
纯化方法:affinity purified by Protein G
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免 疫 原:Recombinant human SP1
SWISS:P08047
Gene ID :6667
Human Gene ID:6667

产品介绍

Profound changes in patterns of gene expression can result from relatively small changes in the concentrations of sequence specific transcription factors. Synergistic interaction between factors bound to different sites within a transcriptional control region is supported by the work of Courey et al. (1989). Sp1 is a sequence specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. SP1 can interact with G/C rich motifs from serotonin receptor promoter. Kadonaga et al. (1987) cloned the human Sp1 cDNA and showed that it has contiguous zinc finger motifs and requires zinc for sequence specific binding to DNA. Altername:Sp1 transcription factor isoform a; TSFP1; TSFP 1; Specificity protein 1; Transcription factor Sp1.
Function:Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes su
Subunit:Interacts with ATF7IP, ATF7IP2, BAHD1, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein. Interacts with HIV-1 Vpr; the interaction is inhibited by SP1 O-glycosylation. Interacts with SV40 VP2/3 proteins. Interacts with SV40 m
Subcellular Location:Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location.
Tissue Specificity:Up-regulated in adenocarcinomas of the stomach (at protein level).
Post-translational modifications:Phosphorylated on multiple serine and threonine residues. Phosphorylation is coupled to ubiquitination, sumoylation and proteolytic processing. Phosphorylation on Ser-59 enhances proteolytic cleavage. Phosphorylation on Ser-7 enhances ubiquitination and p
Similarity:Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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