| Rabbit Anti-FAP antibody |
| 反应物种（预测） |
Rat,Dog,Pig,Cow,Horse |
| 产品应用（已验证） |
IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=3μg/Test,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,免疫学,信号转导,细胞周期蛋白,激酶和磷酸酶 |
| 标签 |
Array |
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Tissue/cell: mouse kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FAPA Polyclonal Antibody, Unconjugated(bs-5758R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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Paraformaldehyde-fixed, paraffin embedded (Human melanoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FAPA) Polyclonal Antibody, Unconjugated (bs-5758R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-FAPA antibody (bs-5758R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_11072621
产品名称：Rabbit Anti-FAP antibody
别名： Prolyl endopeptidase FAP; FAPA; Fibroblast activation protein, alpha; 170 kDa melanoma membrane bound gelatinase; 170 kDa melanoma membrane-bound gelatinase; DPPIV; Fibroblast activation protein alpha; Integral membrane serine protease; SEPR_HUMAN; 170 kD
中文名称：成纤维细胞激活蛋白α抗体
英文名称：Rabbit Anti-FAP antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,细胞膜,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human FAPA
抗原表位：311-410/760
抗原细胞定位：Extracellular
SWISS：Q12884
Gene ID ：2191
Human Gene ID：2191
The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
Function：Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble f
Subunit：Homodimer; homodimerization is required for activity of both plasma membrane and soluble forms. The monomer is inactive. Heterodimer with DPP4. Interacts with PLAUR; the interaction occurs at the cell surface of invadopodia membranes. Interacts with ITGB1
Subcellular Location：Prolyl endopeptidase FAP: Cell surface.Cell membrane. Cell projection, lamellipodium membrane; Single-pass type II membrane protein. Cell projection, invadopodium membrane; Single-pass type II membrane protein. Cell projection, ruffle membrane; Single-pas
Tissue Specificity：Expressed in adipose tissue. Expressed in the dermal fibroblasts in the fetal skin. Expressed in the granulation tissue of healing wounds and on reactive stromal fibroblast in epithelial cancers. Expressed in activated fibroblast-like synoviocytes from in
Post-translational modifications：N-glycosylated.
The N-terminus may be blocked.
Similarity：Belongs to the peptidase S9B family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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